Phytoplasma Firrao et al., 2004
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- Phytoplasma
Abstract
Phytoplasmas are obligate intracellular parasites of plant phloem tissue and of the insect vectors that are involved in their plant-to-plant transmission. Phytoplasmas were discovered in 1967 by Japanese scientists who termed them mycoplasma-like organisms. Since their discovery, phytoplasmas have resisted all attempts at in vitro culture in any cell-free medium; routine cultivation in an artificial medium thus remains a major challenge. Phytoplasmas are characterized by the lack of a cell wall, a pleiomorphic or filamentous shape, a diameter normally less than 1 μm, and a very small genome. Phytoplasmas are pathogens of agriculturally important plants, including coconut, sugarcane, and sandalwood, in which they cause a wide variety of symptoms ranging from mild yellowing to death. Phytoplasmas are most prevalent in tropical and subtropical regions. They are transmitted from plant to plant by vectors (normally sap-sucking insects such as leafhoppers) in which they both survive and replicate.
Control
Phytoplasmas are normally controlled by the breeding and planting of disease-resistant crop varieties (perhaps the most economically viable option) and by the control of insect vectors. Tissue culture can be used to produce healthy clones of phytoplasma-infected plants. Cryotherapy (i.e., the freezing of plant samples in liquid nitrogen) prior to tissue culture increases the probability of producing healthy plants in this manner.Wang et al. (2007) Effective elimination of sweet potato little lead by cryotherapy of shoot tips. Plant Pathology online early edition. Plantibodies targeting phytoplasmas have also been developed. Tetracyclines are bacteriostatic to phytoplasmas. However, disease symptoms reappear in the absence of continuous antibiotic application. Thus, tetracycline is not a viable agricultural control agent, but it is used to protect ornamental coconut trees.Drug for Humans Checks Palm Trees Disease. New York Times, July 19 1983
Detection and diagnosis
Before the molecular era, the diagnosis of phytoplasma-caused diseases was difficult because the organisms could not be cultured. Thus, classical diagnostic techniques, including symptom observation were used. Ultrathin sections of phloem tissue from plants with suspected phytoplasma-infections were also studied. The empirical use of antibiotics such as tetracycline was additionally employed. Molecular diagnostic techniques for phytoplasma detection began to emerge in the 1980s and included enzyme-linked immunosorbent assay (ELISA)-based methods. In the early 1990s, polymerase chain reaction (PCR)-based techniques were developed: these are far more sensitive than ELISAs, and restriction fragment length polymorphism (RFLP) analysis allowed the accurate identification of various phytoplasma strains and species. More recent techniques allow infection levels to be assessed. Both quantitative PCR and bioimaging can effectively quantify phytoplasma titers within plant. In addition, loop-mediated isothermal amplification (a sensitive, simple, and rapid diagnostic method) is now available as a commercial kit allowing all known phytoplasma species to be detected in about 1 h, including the DNA extraction step. Although phytoplasmas have recently been reported to be grown in a specific artificial medium, experimental repetition has yet to be reported.
Effector (virulence) proteins
Many plant pathogens produce virulence factors (i.e., effectors) that modulate or interfere with normal host processes to the benefit of the pathogens. In 2009, a secreted protein, termed “tengu-su inducer” (TENGU; ), was identified from a phytoplasma causing yellowing of onions; this was the first phytoplasmal virulence factor to be described. TENGU induces characteristic symptoms (termed “tengu-su”), including witches' broom and dwarfism. Transgenic expression of TENGU in Arabidopsis plants induced sterility in male and female flowers. TENGU contains a signal peptide at its N-terminus; after cleavage, the mature protein is only 38 amino acids in length. Although phytoplasmas are restricted to phloem, TENGU is transported from phloem to other cells, including those of the apical and axillary meristems. TENGU was suggested to inhibit both auxin- and jasmonic acid-related pathways, thereby affecting plant development. Surprisingly, the N-terminal 11 amino acid region of the mature protein triggers symptom development in Nicotiana benthamiana plants. TENGU undergoes proteolytic processing by a plant serine protease in vivo, suggesting that the N-terminal peptide (i.e., the 11 amino acid fragment) alone induces the observed symptoms. TENGU homologs have been identified in AY-group phytoplasmas. All such homologs undergo processing and can induce symptoms, suggesting that the symptom-inducing mechanism is conserved among TENGU homologs. In 2009, 56 genes for secreted proteins were identified in the genome of aster yellows witches' broom phytoplasma strain (AY-WB); these were named secreted AY-WB proteins (SAPs) and considered effectors. Also in 2009, effector SAP11 was shown to target plant cell nuclei and unload from phloem cells in AY-WB-infected plants. SAP11 was found to induce stem proliferations and changes of leaf shapes of plants; the stem proliferations induced by SAP11 resemble witches' broom symptoms of AY-WB-infected plants. In addition, it was demonstrated that SAP11 interacts with and destabilizes plant class II TCP protein domain transcription factors that leads to shoot proliferations and leaf shape changes. In addition to regulation of plant development, TCPs also control the expression of lipoxygenase genes required for jasmonate biosynthesis. Jasmonate levels are decreased in phytoplasma-infected Arabidopsis plants and plants that transgenically express the AY-WB SAP11 effector. The downregulation of jasmonate production is beneficial to phytoplasmas because jasmonate is involved in plant defenses against herbivorous insects such as leafhoppers. Leafhoppers lay increased numbers of eggs on AY-WB-infected plants, at least in part because of SAP11 production. For example, the leafhopper Macrosteles quadrilineatus laid 30% more eggs on plants that expressing SAP11 transgenically than control plants, and 60% more eggs on plants infected with AY-WB. Phytoplasmas cannot survive in the external environment and are dependent upon insects such as leafhoppers for transmission to new (healthy) plants. Thus, by compromising jasmonate production, SAP11 'encourages' leafhoppers to lay more eggs on phytoplasma-infected plants, thereby ensuring that newly hatched leafhopper nymphs feed upon infected plants to become phytoplasma vectors. SAP11 effectors are identified in a number of divergent phytoplasmas and these effectors also interact with TCPs and modulate plant defenses. SAP11 is the first phytoplasma virulence protein for which plant targets and effector functions (i.e. evidence of benefit for the pathogen) were identified. TCPs were found to be targeted by a number of other pathogen effectors. The AY-WB phytoplasma effector SAP54 was shown to induce virescence and phyllody when expressed in plants and homologs of this effector were found in at least three other phytoplasmas. Two SAP54 homologs, PHYL1 of the onion yellows phytoplasma and PHYL1PnWB of the peanut witches' broom phytoplasma, also induce phyllody-like floral abnormalities. These results suggest that PHYL1, SAP54, and their homologs form a phyllody-inducing gene family, the members of which are termed phyllogens. MADS-box transcription factors (MTFs) of the ABCE model play critical roles in floral organ development in Arabidopsis. Phyllogens interact directly with class A and class E MTFs, inducing protein degradation in a ubiquitin/proteasome-dependent manner that, at least for SAP54, is dependent on interactions with the proteasome shuttle factor RAD23. Interestingly, RAD23 mutants do not show phyllody when infected with phytoplasma indicating that RAD23 proteins are susceptibility factors; i.e. phytoplasmas and SAP54 require these plant proteins to induce phyllody symptoms. The accumulation of mRNAs encoding class B MTFs, the transcription of which is positively regulated by class A and class E MTFs, is drastically decreased in Arabidopsis constitutively expressing PHYL1. Phyllogens induce abnormal floral organ development by inhibiting the functions of these MTFs. RAD23 proteins are also required for promoting leafhopper vector egg laying on plants that express SAP54 and are infected with AY-WB phytoplasma.
Gallery
Image:Vitis vinifera phytoplasma.jpg|A [[grape]] vine with "[[Candidatus Phytoplasma solani|bois noir]]" phytoplasma disease Image:Flavescence dorée 3.jpg|A grape vine with "[[flavescence dorée]]" phytoplasma disease Image:Lethalyellowingpalms.jpg|Coconut palms dying of [[lethal yellowing]] disease Image:Asteryellowsmarigold.jpg|Symptoms of [[aster yellows]] on [[Tagetes|marigold]] Image:TPWB1.tif|''[[Tephrosia purpurea]]'' witches' broom Image:Elmphloemnecrosis.jpg|Symptoms of [[elm]] [[Elm yellows|phloem necrosis phytoplasma]] Image:Brinjal Little leaf phytoplasma.jpg|[[Eggplant|Brinjal]] Little leaf phytoplasma Image:Ashyellows.jpg|Trees dying of [[Fraxinus|ash]] yellows phytoplasma Image:Parthenium hysterophorus witches' broom phytoplasma.jpg|[[Parthenium hysterophorus]] showing symptoms of [[witches' broom]] Image:Phyllody on Cosmos.jpg|[[Phyllody]] caused by phytoplasma infection on [[Cosmos (plant)|''Cosmos'' spp.]] Image:Little leaf disease of Cleome viscosa.jpg|Little leaf disease of [[Cleome viscosa]] Image:Sweetpotatoelittleleaf.JPG|Symptoms of sweet potato little leaf phytoplasma on ''[[Catharanthus roseus]]'' Image:Phyllody on Solidago.JPG|Phyllody of [[Solidago|goldenrod]] Image:Soybean Phytoplasm.jpg|[[Soybean]] Phytoplasma Image:China Aster Phyllody Phytoplasma.jpg|A flower of [[Callistephus|China Aster]] showing [[phyllody]] symptoms Image:Amit Yadav SCGS 040620093043.jpg|[[Sugarcane grassy shoot disease]] Image:Coconutlethalyellowing.jpg|A palm tree dying of lethal yellowing phytoplasma File:Sudden Decline.jpg|A [[Cordyline australis|cabbage tree]] killed by ''[[Phytoplasma australiense]]'' Image:Bamboo Phytoplasma.jpg|Witches' broom on [[bamboo]] (''[[Dendrocalamus strictus]]'') File:Trillium_grandiflorum_at_the_North_Walker_Woods1.jpg|''[[Trillium grandiflorum]]'' with [[Virescence|virescent]] petals
Genetics
The genomes of four phytoplasmas have been sequenced: "onion yellows", "aster yellows witches' broom" (Candidatus Phytoplasma asteris), Ca. Phytoplasma australiense, and Ca. Phytoplasma mali. Phytoplasmas have very small genomes, with extremely low GC content (sometimes as little as 23%, which is thought to be the lower threshold for a viable genome).Dikinson, M. Molecular Plant Pathology (2003) BIOS Scientific Publishers In fact, the Bermuda grass white-leaf phytoplasma (Candidatus Phytoplasma cynodontis) has a genome size of only 530 kb, one of the smallest known genomes of all living organisms. The larger phytoplasma genomes are around 1350 kb in size. The small genome size of phytoplasma is attributable to reductive evolution from Bacillus/Clostridium ancestors. Phytoplasmas have lost ≥75% of their original genes, and can thus no longer survive outside of insects or plant phloem. Some phytoplasmas contain extrachromosomal DNA such as plasmids. Despite their small genomes, many predicted phytoplasma genes are present in multiple copies. Phytoplasmas lack many genes encoding standard metabolic functions and have no functioning homologous recombination pathway, but they do have a sec transport pathway. Many phytoplasmas contain two rRNA operons. Unlike other Mollicutes, the triplet code of UGA is used as a stop codon in phytoplasmas. Phytoplasma genomes contain large numbers of transposons and insertion sequences and also contain a unique family of repetitive extragenic palindromes termed PhREPS for which no role is known. However, it is theorized that the stem-loop structures in PhREPS play a role in transcription termination or genome stability.
History
References to diseases now known to be caused by phytoplasmas can be found as far back as 1603 (mulberry dwarf disease in Japan). Such diseases were originally thought to be caused by viruses, which, like phytoplasmas, require insect vectors, and cannot be cultured. Viral and phytoplasmic infections share some symptoms. In 1967, phytoplasmas were discovered in ultrathin sections of plant phloem tissue and were termed mycoplasma-like organisms due to their physiological resemblance. The organisms were renamed phytoplasmas in 1994, at the 10th Congress of the International Organization for Mycoplasmology.
Morphology
Phytoplasmas are Mollicutes, which are bound by a triple-layered membrane, rather than a cell wall. The phytoplasma cell membranes studied to date usually contain a single immunodominant protein of unknown function that constitutes most of the protein in the membrane. A typical phytoplasma is pleiomorphic or filamentous in shape and is less than 1 μm in diameter. Like other prokaryotes, phytoplasmic DNA is distributed throughout the cytoplasm, instead of being concentrated in a nucleus.
Species
, the following names and type strains are from LPSN,, the List of Prokaryotic names with Standing in Nomenclature. The associated diseases and 16Sr group-subgroup classifications are from various sources.
'Candidatus Phytoplasma' species Species name Associated disease Type strain 16Sr group-subgroup Candidatus Phytoplasma allocasuarinae Marcone et al. 2004 Allocasuarina yellows AlloY 16SrXXXIII-A Candidatus Phytoplasma americanum Lee et al. 2006 American potato purple top wilt APPTW12-NE; PPT12-NE 16SrXVIII-A Candidatus Phytoplasma asteris Lee et al. 2004 Aster yellows MIAY; OAY 16SrI-B Candidatus Phytoplasma australamericanum corrig. Davis et al. 2012 ( Davis et al. 2012) Passionfruit witches' broom PassWB-Br3; PassWB-Br3R 16SrVI-I Candidatus Phytoplasma australasiaticum corrig. White et al. 1998 ( White et al. 1998) Papaya mosaic PpYC 16SrII-D Candidatus Phytoplasma australiense Davis et al. 1997 Australian grapevine yellows none 16SrXII-B Candidatus Phytoplasma balanitis corrig. Win et al. 2013 ( Win et al. 2013) Balanites witches' broom none 16SrV-F Candidatus Phytoplasma brasiliense Montano et al. 2001 Hibiscus witches' broom HibWB26 16SrXV-A Candidatus Phytoplasma caricae Arocha et al. 2005 Papaya bunchy top PAY 16SrXVII-A Candidatus Phytoplasma castaneae Jung et al. 2002 Chestnut witches' broom CnWB 16SrXIX-A Candidatus Phytoplasma cirsii Šafárová et al. 2016 Cirsium yellows and stunting CirYS 16SrXI-E Candidatus Phytoplasma citri corrig. Zreik et al. 1995 ( Zreik et al. 1995) Lime witches' broom WBDL 16SrII-B Candidatus Phytoplasma cocosnigeriae Firrao et al. 2004 Candidatus Phytoplasma cocostanzaniae Firrao et al. 2004 Candidatus Phytoplasma convolvuli Martini et al. 2012 Bindweed yellows BY-S57/11 16SrXII-H Candidatus Phytoplasma costaricanum Lee et al. 2011 Soybean stunt SoyST1c1 16SrXXXI-A Candidatus Phytoplasma cynodontis Marcone et al. 2004 Bermuda grass white leaf BGWL-C1 16SrXIV-A Candidatus Phytoplasma dypsidis Jones et al. 2021 RID7692 16SrIV-? Candidatus Phytoplasma fragariae Valiunas et al. 2006 Strawberry yellows StrawY; StrawYR 16SrXII-E Candidatus Phytoplasma fraxini Griffiths et al. 1999 Ash yellows AshY1; Ashy lT 16SrVII-A Candidatus Phytoplasma graminis Arocha et al. 2005 Sugarcane yellow leaf SCYLP 16SrXVI-A Candidatus Phytoplasma hispanicum Davis et al. 2016 Mexican periwinkle virescence MPV; MPVR 16SrXIII-A Candidatus Phytoplasma japonicum Sawayanagi et al. 1999 Japanese hydrangea phyllody none 16SrXII-D Candidatus Phytoplasma luffae Davis et al. 2017 Loofah witches' broom LfWB; LfWBR 16SrVIII-A Candidatus Phytoplasma lycopersici Arocha et al. 2007 Tomato 'brote grande' THP 16SrI-Y Candidatus Phytoplasma malaysianum Nejat et al. 2013 Malaysian periwinkle virescence MaPV; MaPVR 16SrXXXII-A Candidatus Phytoplasma mali Seemüller and Schneider 2004 Apple proliferation AP15 16SrX-A Candidatus Phytoplasma meliae Fernández et al. 2016 Chinaberry yellowing ChTY-Mo3 16SrXIII-G Candidatus Phytoplasma noviguineense Miyazaki et al. 2018 Bogia coconut syndrome BCS-Bo; BCS-BoR Candidatus Phytoplasma omanense Al-Saady et al. 2008 Cassia witches' broom IM-1 16SrXXIX-A Candidatus Phytoplasma oryzae Jung et al. 2003 Rice yellow dwarf RYD-Th 16SrXI-A Candidatus Phytoplasma palmae Firrao et al. 2004 Palm lethal yellowing 16SrIV-D Candidatus Phytoplasma palmicola Harrison et al. 2014 Coconut lethal yellowing LYDM-178; LYDM-178R 16SrXXII-A Candidatus Phytoplasma phoenicium Verdin et al. 2003 Almond witches' broom A4 16SrIX-B Candidatus Phytoplasma pini Schneider et al. 2005 Pine witches' broom Pin127S; Pin127SR 16SrXXI-A Candidatus Phytoplasma pruni Davis et al. 2013 Peach X-disease PX11Ct1; PX11CT1R 16SrIII-A Candidatus Phytoplasma prunorum Seemüller and Schneider 2004 European stone fruit yellows ESFY-G1 16SrX-B Candidatus Phytoplasma pyri Seemüller and Schneider 2004 Pear decline PD1 16SrX-C Candidatus Phytoplasma rhamni Marcone et al. 2004 Rhamnus witches' broom BAWB; BWB 16SrXX-A Candidatus Phytoplasma rubi Malembic-Maher et al. 2011 Rubus stunt RuS 16SrV-E Sugarcane Grassy Shoot Disease Kirdat et al. 2021 Sugarcane Grassy Shoot Disease SCGS 16SrXI-B Candidatus Phytoplasma solani Quaglino et al. 2013 Stolbur STOL; STOL11R 16SrXII-A Candidatus Phytoplasma spartii Marcone et al. 2004 Spartium witches' broom SpaWB 16SrX-D Candidatus Phytoplasma stylosanthis Rodrigues-Jardim et al. 2021 VPRI 43683 16SrXXXVII-A Candidatus Phytoplasma tamaricis Zhao et al. 2009 Salt cedar witches' broom SCWB1; SCWB1R 16SrXXX-A Candidatus Phytoplasma trifolii Hiruki and Wang 2004 Clover proliferation CP 16SrVI-A Candidatus Phytoplasma tritici Zhao et al. 2021 WBD R 16SrI-C Candidatus Phytoplasma ulmi Lee et al. 2004 Elm yellows EY1 16SrV-A Candidatus Phytoplasma vitis Firrao et al. 2004 none 16SrV-? Candidatus Phytoplasma wodyetiae Naderali et al. 2017 Foxtail palm yellow decline Bangi-2; FPYD Bangi-2R 16SrXXXVI-A Candidatus Phytoplasma ziziphi Jung et al. 2003 Jujube witches' broom JWB; JWB-Ky 16SrV-B
'Candidatus Phytoplasma' species Species name Associated disease Type strain 16Sr group-subgroup Candidatus Phytoplasma allocasuarinae Marcone et al. 2004 Allocasuarina yellows AlloY 16SrXXXIII-A Candidatus Phytoplasma americanum Lee et al. 2006 American potato purple top wilt APPTW12-NE; PPT12-NE 16SrXVIII-A Candidatus Phytoplasma asteris Lee et al. 2004 Aster yellows MIAY; OAY 16SrI-B Candidatus Phytoplasma australamericanum corrig. Davis et al. 2012 ( Davis et al. 2012) Passionfruit witches' broom PassWB-Br3; PassWB-Br3R 16SrVI-I Candidatus Phytoplasma australasiaticum corrig. White et al. 1998 ( White et al. 1998) Papaya mosaic PpYC 16SrII-D Candidatus Phytoplasma australiense Davis et al. 1997 Australian grapevine yellows none 16SrXII-B Candidatus Phytoplasma balanitis corrig. Win et al. 2013 ( Win et al. 2013) Balanites witches' broom none 16SrV-F Candidatus Phytoplasma brasiliense Montano et al. 2001 Hibiscus witches' broom HibWB26 16SrXV-A Candidatus Phytoplasma caricae Arocha et al. 2005 Papaya bunchy top PAY 16SrXVII-A Candidatus Phytoplasma castaneae Jung et al. 2002 Chestnut witches' broom CnWB 16SrXIX-A Candidatus Phytoplasma cirsii Šafárová et al. 2016 Cirsium yellows and stunting CirYS 16SrXI-E Candidatus Phytoplasma citri corrig. Zreik et al. 1995 ( Zreik et al. 1995) Lime witches' broom WBDL 16SrII-B Candidatus Phytoplasma cocosnigeriae Firrao et al. 2004 Candidatus Phytoplasma cocostanzaniae Firrao et al. 2004 Candidatus Phytoplasma convolvuli Martini et al. 2012 Bindweed yellows BY-S57/11 16SrXII-H Candidatus Phytoplasma costaricanum Lee et al. 2011 Soybean stunt SoyST1c1 16SrXXXI-A Candidatus Phytoplasma cynodontis Marcone et al. 2004 Bermuda grass white leaf BGWL-C1 16SrXIV-A Candidatus Phytoplasma dypsidis Jones et al. 2021 RID7692 16SrIV-? Candidatus Phytoplasma fragariae Valiunas et al. 2006 Strawberry yellows StrawY; StrawYR 16SrXII-E Candidatus Phytoplasma fraxini Griffiths et al. 1999 Ash yellows AshY1; Ashy lT 16SrVII-A Candidatus Phytoplasma graminis Arocha et al. 2005 Sugarcane yellow leaf SCYLP 16SrXVI-A Candidatus Phytoplasma hispanicum Davis et al. 2016 Mexican periwinkle virescence MPV; MPVR 16SrXIII-A Candidatus Phytoplasma japonicum Sawayanagi et al. 1999 Japanese hydrangea phyllody none 16SrXII-D Candidatus Phytoplasma luffae Davis et al. 2017 Loofah witches' broom LfWB; LfWBR 16SrVIII-A Candidatus Phytoplasma lycopersici Arocha et al. 2007 Tomato 'brote grande' THP 16SrI-Y Candidatus Phytoplasma malaysianum Nejat et al. 2013 Malaysian periwinkle virescence MaPV; MaPVR 16SrXXXII-A Candidatus Phytoplasma mali Seemüller and Schneider 2004 Apple proliferation AP15 16SrX-A Candidatus Phytoplasma meliae Fernández et al. 2016 Chinaberry yellowing ChTY-Mo3 16SrXIII-G Candidatus Phytoplasma noviguineense Miyazaki et al. 2018 Bogia coconut syndrome BCS-Bo; BCS-BoR Candidatus Phytoplasma omanense Al-Saady et al. 2008 Cassia witches' broom IM-1 16SrXXIX-A Candidatus Phytoplasma oryzae Jung et al. 2003 Rice yellow dwarf RYD-Th 16SrXI-A Candidatus Phytoplasma palmae Firrao et al. 2004 Palm lethal yellowing 16SrIV-D Candidatus Phytoplasma palmicola Harrison et al. 2014 Coconut lethal yellowing LYDM-178; LYDM-178R 16SrXXII-A Candidatus Phytoplasma phoenicium Verdin et al. 2003 Almond witches' broom A4 16SrIX-B Candidatus Phytoplasma pini Schneider et al. 2005 Pine witches' broom Pin127S; Pin127SR 16SrXXI-A Candidatus Phytoplasma pruni Davis et al. 2013 Peach X-disease PX11Ct1; PX11CT1R 16SrIII-A Candidatus Phytoplasma prunorum Seemüller and Schneider 2004 European stone fruit yellows ESFY-G1 16SrX-B Candidatus Phytoplasma pyri Seemüller and Schneider 2004 Pear decline PD1 16SrX-C Candidatus Phytoplasma rhamni Marcone et al. 2004 Rhamnus witches' broom BAWB; BWB 16SrXX-A Candidatus Phytoplasma rubi Malembic-Maher et al. 2011 Rubus stunt RuS 16SrV-E Sugarcane Grassy Shoot Disease Kirdat et al. 2021 Sugarcane Grassy Shoot Disease SCGS 16SrXI-B Candidatus Phytoplasma solani Quaglino et al. 2013 Stolbur STOL; STOL11R 16SrXII-A Candidatus Phytoplasma spartii Marcone et al. 2004 Spartium witches' broom SpaWB 16SrX-D Candidatus Phytoplasma stylosanthis Rodrigues-Jardim et al. 2021 VPRI 43683 16SrXXXVII-A Candidatus Phytoplasma tamaricis Zhao et al. 2009 Salt cedar witches' broom SCWB1; SCWB1R 16SrXXX-A Candidatus Phytoplasma trifolii Hiruki and Wang 2004 Clover proliferation CP 16SrVI-A Candidatus Phytoplasma tritici Zhao et al. 2021 WBD R 16SrI-C Candidatus Phytoplasma ulmi Lee et al. 2004 Elm yellows EY1 16SrV-A Candidatus Phytoplasma vitis Firrao et al. 2004 none 16SrV-? Candidatus Phytoplasma wodyetiae Naderali et al. 2017 Foxtail palm yellow decline Bangi-2; FPYD Bangi-2R 16SrXXXVI-A Candidatus Phytoplasma ziziphi Jung et al. 2003 Jujube witches' broom JWB; JWB-Ky 16SrV-B
Symptoms
Phytoplasmas can infect and cause various symptoms in more than 700 plant species. One characteristic symptom is abnormal floral organ development including phyllody, (i.e., the production of leaf-like structures in place of flowers) and virescence (i.e., the development of green flowers attributable to a loss of pigment by petal cells). Phytoplasma-harboring flowering plants may nevertheless be sterile. The expression of genes involved in maintaining the apical meristem or in the development of floral organs is altered in the morphologically affected floral organs of phytoplasma-infected plants. A phytoplasma infection often triggers leaf yellowing, probably due to the presence of phytoplasma cells in phloem, which can affect phloem function and carbohydrate transport, inhibit chlorophyll biosynthesis, and trigger chlorophyll breakdown. These symptoms may be attributable to stress caused by the infection rather than a specific pathogenetic process. Many phytoplasma-infected plants develop a bushy or "witches' broom" appearance due to changes in their normal growth patterns. Most plants exhibit apical dominance but infection can trigger the proliferation of axillary (side) shoots and a reduction in internode size. Such symptoms are actually useful in the commercial production of poinsettias. Infection triggers more axillary shoot production; the poinsettia plants thus produce more than a single flower.
Taxonomy
Phytoplasmas belong to the monotypic order Acholeplasmatales. In 1992, the Subcommittee on the Taxonomy of Mollicutes proposed the use of 'Phytoplasma' rather than 'mycoplasma-like organisms' 'for reference to the phytopathogenic mollicutes'.Subcommittee on the Taxonomy of Mollicutes. Minutes of the Interim Meetings, 1 and 2 August, 1992, Ames, Iowa Int. J. Syst. Bacteriol. April 1993, p. 394–397; Vol. 43, No. 2 (see minutes 10 and 25) In 2004, the generic name Phytoplasma was adopted and is currently of Candidatus (Ca.) status (used for bacteria that cannot be cultured). Phytoplasma taxonomy is complicated because the organisms cannot be cultured; methods normally used to classify prokaryotes are thus not available. Phytoplasma taxonomic groups are based on differences in fragment sizes produced by restriction digests of 16S ribosomal RNA gene sequences (RFLPs) or by comparisons of DNA sequences from 16s/23s spacer regions. The actual number of taxonomic groups remains unclear; recent work on computer-simulated restriction digests of the 16Sr gene suggested up to 28 groups, whereas others have proposed fewer groups with more subgroups. Each group includes at least one Ca. Phytoplasma species, characterized by distinctive biological, phytopathological, and genetic properties.
Transmission
Movement between plants Phytoplasmas are spread principally by insects of the families Cicadellidae (leafhoppers), Fulgoridae (planthoppers), and Psyllidae (jumping plant lice), which feed on the phloem of infected plants, ingesting phytoplasmas and transmitting them to the next plant on which they feed. Thus, the host range of phytoplasmas is strongly dependent upon that of the insect vector. Phytoplasmas contain a major antigenic protein constituting most of the cell surface protein. This protein associates with insect microfilament complexes and is believed to control insect-phytoplasma interactions. Phytoplasmas can overwinter in insect vectors or perennial plants. Phytoplasmas can have varying effects on their insect hosts; examples of both reduced and increased fitness have been noted. Phytoplasmas enter the insect body through the stylet, pass through the intestine, and then move to the hemolymph and colonize the salivary glands: the entire process can take up to 3 weeks. Once established in an insect host, phytoplasmas are found in most major organs. The time between ingestion by the insect and attainment of an infectious titer in the salivary glands is termed the latency period. Phytoplasmas can also be spread via dodders (Cuscuta) or by vegetative propagation such as the grafting of infected plant tissue onto a healthy plant.
Movement within plants Phytoplasmas move within phloem from a source to a sink, and can pass through sieve tube element. However, as phytoplasmas spread more slowly than solutes, and for other reasons, passive translocation within plants is thought to be unimportant
Movement within plants Phytoplasmas move within phloem from a source to a sink, and can pass through sieve tube element. However, as phytoplasmas spread more slowly than solutes, and for other reasons, passive translocation within plants is thought to be unimportant