The Fungarium of Elena Zvyagina
Citation
Zvyagina E (2024). The Fungarium of Elena Zvyagina. Version 1.74. Yugra State University Biological Collection (YSU BC). Occurrence dataset https://doi.org/10.15468/xxymuf accessed via GBIF.org on 2024-12-09.Description
Elena Zvyagina's personal collection of fungi collected as a result of field research in various regions of Russia. At the moment, the dataset is loaded mainly with specimens collected in North Karelia, Western Siberia, and the Russian Far East. The collection dataset also includes parts of specimens loaned by various herbaria and personal collections in the course of work on Boletaceae, even if these specimens were returned to the herbarium. The classical method of collection and preservation of dry specimens of macrofungi is used to store the material. A large part of collection has been sequenced and has attached sequence material. The collection is stored at the White Sea Biological Station of Moscow State University (186672, Russia, Republic of Karelia, poselok Primorsky).
Sampling Description
Study Extent
Collections were made mostly in boreal zone of Eurasia during the study of fungal diversity of taiga forests, subpolar mountains ecosystems and taxonomy of Boletales order.Sampling
Specimens were photographed in situ for macromorphological features. Fading out features like smell and taste were described in field. Sporocarps were dried in the mushroom dryer under 40-50 °C. Dried specimens are stored in Ziplocs and paper envelopes.Quality Control
Most of the specimens are providet with photografs. DNA sequences uploaded to GenBank. Specimens are available by the personal requestMethod steps
- Forays were undertaken randomly
- Specimens were photographed in situ for macromorphological features
- Fading out features like smell and taste were described in field
- Sporocarps were extracted from substrate and putted into aluminum foil
- Sporocarps were dried in the mushroom dryer under 40-50 °C
- Dried specimens are stored in Ziplocs and paper envelopes
- For amplification of the ITS region the primers ITS1-F, ITS4-B (Gardes, Bruns, 1993) were used
- LSU (28S) was amplified with LR0R, LR5 (Vilgalys, Hester, 1990) pair of primers, TEF1 with EF1-983F and EF1-1567R (Rehner, Buckley, 2005)
- Amplified products were sequenced using BigDyeH Terminator 3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, California). 10) The sequences were assembled in CodonCode Aligner V.9.0.1 (CodonCode Corporation)
Taxonomic Coverages
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fungirank: kingdom
Geographic Coverages
Bibliographic Citations
- Gardes, M., Bruns, T.D. (1993) ITS primers with enhanced specifity for Basidiomycetes: application to identification of mycorrhizae and rusts. Mol. Ecol. 2: 113–118. -
- Vilgalys, R., Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4239–4246. -
- Rehner, S.A., Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: Evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97: 84–98. -
Contacts
Elena Zvyaginaoriginator
position: researcher
Yugra State University
186672, Russia, Republic of Karelia, pos. Primorsky
Khanty-Mansiysk
186672
Republic of Karelia
RU
email: mycena@yandex.ru
userId: https://orcid.org/0000-0003-2063-4847
Elena Zvyagina
metadata author
position: researcher
Yugra State University
186672, Russia, Republic of Karelia, pos. Primorsky
Khanty-Mansiysk
186672
Republic of Karelia
RU
email: mycena@yandex.ru
userId: https://orcid.org/0000-0003-2063-4847
Elena Zvyagina
administrative point of contact
position: researcher
Yugra State University
186672, Russia, Republic of Karelia, pos. Primorsky
Khanty-Mansiysk
186672
Republic of Karelia
RU
email: mycena@yandex.ru
userId: https://orcid.org/0000-0003-2063-4847