The Fungarium of Elena Zvyagina

Study Extent

Collections were made mostly in boreal zone of Eurasia during the study of fungal diversity of taiga forests, subpolar mountains ecosystems and taxonomy of Boletales order.

Sampling

Specimens were photographed in situ for macromorphological features. Fading out features like smell and taste were described in field. Sporocarps were dried in the mushroom dryer under 40-50 °C. Dried specimens are stored in Ziplocs and paper envelopes.

Quality Control

Most of the specimens are providet with photografs. DNA sequences uploaded to GenBank. Specimens are available by the personal request

Method steps

1. Forays were undertaken randomly
2. Specimens were photographed in situ for macromorphological features
3. Fading out features like smell and taste were described in field
4. Sporocarps were extracted from substrate and putted into aluminum foil
5. Sporocarps were dried in the mushroom dryer under 40-50 °C
6. Dried specimens are stored in Ziplocs and paper envelopes
7. For amplification of the ITS region the primers ITS1-F, ITS4-B (Gardes, Bruns, 1993) were used
8. LSU (28S) was amplified with LR0R, LR5 (Vilgalys, Hester, 1990) pair of primers, TEF1 with EF1-983F and EF1-1567R (Rehner, Buckley, 2005)
9. Amplified products were sequenced using BigDyeH Terminator 3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, California). 10) The sequences were assembled in CodonCode Aligner V.9.0.1 (CodonCode Corporation)

1. fungi
   rank: kingdom

The geography of the collection covers mainly Northern West Siberia and other regions sampled during the field work in several regions of Russia.