Microbial diversity in four Mediterranean irciniid sponges
Citation
Skaraki K, Dailianis T, Pavloudi C (2023). Microbial diversity in four Mediterranean irciniid sponges. Hellenic Center for Marine Research. Occurrence dataset https://doi.org/10.15468/3uxuba accessed via GBIF.org on 2024-12-12.Description
These data are from sponge samples collected in Crete, Greece. The examined sponges all belong to Demospongiae (Class); Keratosa (Subclass); Dictyoceratida (Order); Irciniidae (Family). Four sponge species were examined: Ircinia oros (Schmidt, 1864), Ircinia variabilis (Schmidt, 1862), Sarcotragus spinosulus Schmidt, 1862 and Sarcotragus fasciculatus (Pallas, 1766). They have undergone metabarcoding for the V5-V6 region of the 16S rRNA. Samples were collected by scuba diving at depths between 6-12m from two sampling sites of rocky formations at the northern coast of Crete (Cretan Sea, Eastern Mediterranean). All the raw sequence files of this study were submitted to the European Nucleotide Archive (ENA) with the study accession number PRJEB23640 (available at http://www.ebi.ac.uk/ena/data/view/PRJEB23640)Sampling Description
Method steps
- raw data were denoised using the software package AmpliconNoise (Quince et al., 2011) by removing pyrosequencing noise and correcting for putative PCR errors
- the Perseus algorithm was used to check for chimeras and the denoised reads were clustered to Operational Taxonomic Units (OTUs), with a complete linkage algorithm on a 97% sequence identity level (Quince et al., 2011),
- taxonomy assignment was performed using GTDB r207 (Parks et al., 2022) using the IDTAXA Classifier (Murali, Bhargava & Wright, 2018) and a confidence threshold ≥60% (cautious).
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
Contacts
Katerina Skarakioriginator
position: Biologist
Laboratory of Biodiversity, Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research
GR
email: kskaraki@gmail.com
userId: http://orcid.org/0000-0003-1989-8458
Thanos Dailianis
originator
position: Biologist
Laboratory of Biodiversity, Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research
GR
email: thanosd@hcmr.gr
userId: http://orcid.org/0000-0002-4102-9331
Christina Pavloudi
originator
position: Biologist
Laboratory of Biodiversity, Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research
GR
email: cpavloud@hcmr.gr
userId: http://orcid.org/0000-0001-5106-6067
Christina Pavloudi
administrative point of contact
position: Biologist
Laboratory of Biodiversity, Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research
GR
email: cpavloud@hcmr.gr
userId: http://orcid.org/0000-0001-5106-6067