croplm
Citation
Kermek D, Pischiutta N, Hlebec D (2024). croplm. Biodiversity Data Journal. Occurrence dataset https://doi.org/10.15468/7rydnq accessed via GBIF.org on 2024-12-10.Description
Stoneflies of Medvednica Nature Park: genetic diversity and morphological variabilitySampling Description
Study Extent
Specimens were collected at eight sampling sites in Medvednica Nature Park.Sampling
Adults were collected using an entomological net while larvae were collected by hand under stones and branches.Quality Control
All collected individuals were preserved in ethanol. Morphological determination of adults was conducted using a ZEISS SteREO Discovery V20 stereo microscope along with various identification keys. Due to the lack of identification keys the larvae were not determined to the species level. Larval identification was carried out by comparing their sequences with sequences available in databases.Method steps
- Total genomic DNA was isolated from the tissues of one to three legs of adults and larvae using the GenElute Mammalian Genomic DNA Miniprep Kit. Chromatograms were analyzed and edited in Geneious Prime. Misread nucleotides were manually corrected. The presence of stop codons in all sequences was checked using Mesquite. Poor quality sequences and those containing stop codons were excluded from further analyses. For phylogeographic analyses, we selected species with a broader distribution and a larger number of COI sequences available in the BOLD and GenBank databases.
Taxonomic Coverages
Stoneflies
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Plecopteracommon name: Stoneflies rank: order
Geographic Coverages
Croatia (Medvednica Mt.)
Bibliographic Citations
Contacts
Dora Kermekoriginator
position: Student
Faculty of Science, University of Zagreb
HR
Nikola Pischiutta
originator
position: Student
Faculty of Science, University of Zagreb
Dora Hlebec
originator
position: Assistant
Faculty of Science, University of Zagreb
userId: http://orcid.org/0000-0001-5967-864X
Dora Hlebec
metadata author
position: Assistant
Faculty of Science, University of Zagreb
Horvatovac 102a
Zagreb
10000
HR
email: dora.hlebec@gmail.com
userId: http://scholar.google.com/citations?user=0000-0001-5967-864X
Iva Mihoci
curator
position: Curator
Croatian Natural History Museum
Demetrova 1
Zagreb
10000
HR
Dora Hlebec
administrative point of contact
position: Assistant
Faculty of Science, University of Zagreb
Horvatovac 102a
Zagreb
10000
HR
email: dora.hlebec@gmail.com
userId: http://scholar.google.com/citations?user=0000-0001-5967-864X