The InBIO Barcoding Initiative Database: DNA barcodes of Iberian Trichoptera 01
Citation
Martín L, Ferreira S, Martínez J, González M A, Corley M, Grosso-Silva J M, Quaglietta L, Sousa P, Beja P, Ferreira S (2023). The InBIO Barcoding Initiative Database: DNA barcodes of Iberian Trichoptera 01. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/hsa3kw accessed via GBIF.org on 2024-10-04.Description
The dataset contains 438 records of Trichoptera species from 22 of the 23 families known from the Iberian Peninsula. Specimens were collected between 1975 to 2018 in Portugal, Spain and France (Paupério et al., 2023). Specimens have been identified to species or subspecies level, in a total of 141 species representing 37% of the Caddisflies known for the Iberian Peninsula. Specimens were captured during fieldwork directed specifically for the sampling of Trichoptera using different methodologies and stored in 96% ethanol. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. Sequencing of the 658 bp COI DNA barcode was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases. Specimens are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources, Portugal) or in the collection Marcos A. González at the University of Santiago de Compostela (Spain). All DNA extracts are deposited in the IBI collection.Purpose
The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.Sampling Description
Study Extent
Portugal, Spain and France.Sampling
The studied material was collected in 142 different localities, 74 in Spain, 66 in Portugal and two in France. Sampling was conducted between 1975 and 2018, although 62% of the records were made between 2015 and 2017. Specimens were collected by direct search and individual netting of specimens, by sweeping the vegetation with a net or were directly collected at light traps, using both UV and mercury vapor lights. Captured specimens deposited in the IBI reference collection were preserved in 96% alcohol.Quality Control
For all DNA barcoded specimens, their morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).Method steps
- Specimens were collected in 142 different localities in the Iberian Peninsula and France. Field work was carried out between 1975 and 2018, with 62% of the records made between the years 2015 and 2017.
- Specimens were collected during fieldwork by direct search and individual netting of specimens, by sweeping the vegetation or were directly collected at light traps (using both UV and mercury vapor lights) and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources, Portugal) or in the collection Marcos A. González at the University of Santiago de Compostela (Spain).
- All specimens were morphologically identified using the available literature.
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. All DNA extracts were deposited in the IBI collection.
- All sequences in the dataset were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).
Taxonomic Coverages
This dataset is composed of data relating to 438 Trichoptera specimens. All specimens were determined to species level except for two specimens that were identified to genus level. Overall, 141 species are represented in the dataset. Four species have been further identified to subspecies level: Wormaldia variegata mattheyi Schmid, 1952, Philopotamus montanus caurelensis González & Terra, 1979, Wormaldia variegata mattheyi Schmid, 1952, Rhyacophila dorsalis albarracina Malicky, 2002 and Rhyacophila mocsaryi tredosensis Schmid, 1952.
The species represented in the dataset belong to 56 genera unevenly distributed, with the families Limnephilidae (11 genera, 19.6% of the total), Leptoceridae (8 genera, 14.3%), Glossosomatidae, Hydroptilidae and Psychomyiidae (each with 4 genera or 7.1% of the total) accounting for 55% of the genera represented in the dataset. Three families account for 43% of the species represented in the dataset, Limnephilidae and Rhyacophilidae (each with 23 species or 16.3% of the total) and Hydropsychidae (15 species, 10.6%).
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Animaliarank: kingdom
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Arthropodarank: phylum
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Insectarank: class
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Trichoptera Family:rank: order
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Apataniidaerank: family
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Beraeidaerank: family
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Brachycentridaerank: family
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Calamoceratidaerank: family
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Ecnomidaerank: family
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Glossosomatidaerank: family
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Goeridaerank: family
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Helicopsychidaerank: family
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Hydropsychidaerank: family
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Hydroptilidaerank: family
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Lepidostomatidaerank: family
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Leptoceridaerank: family
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Limnephilidaerank: family
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Odontoceridaerank: family
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Philopotamidaerank: family
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Phryganeidaerank: family
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Polycentropodidaerank: family
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Psychomyiidaerank: family
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Ptilocolepidaerank: family
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Rhyacophilidaerank: family
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Sericostomatidaerank: family
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Uenoidaerank: family
Geographic Coverages
Iberian Peninsula, France
Bibliographic Citations
- Pauperio, J., Gonzalez, L.M., Martinez, J., González, M.A., Martins, F.M., Veríssimo, J., Puppo, P., Pinto. J., Chaves, C., Pinho, C.J., Grosso-Silva, J.M., Quaglietta, L., Silva, T.L.L., Sousa, P., Alves, P.C., Fonseca, N., Beja, P. & Ferreira, S. (2023). The InBIO barcoding initiative database: DNA barcodes of Iberian Trichoptera, documenting biodiversity for freshwater biomonitoring in a Mediterranean hotspot. Biodiversity Data Journal 11: e97484. https://doi.org/10.3897/BDJ.11.e97484 -
Contacts
Luis Martínoriginator
position: Post-Doc Researcher
Department Zoología, Antropología Física & Genética, Faculty of Biology, University of Santiago de Compostela
Santiago de Compostela
Galicia
ES
userId: https://www.linkedin.com/profile/view?id=luismartíngonzález
Sónia´ Ferreira
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966
Jesús Martínez
originator
position: Post-Doc Researcher
Department Zoología, Antropología Física & Genética, Faculty of Biology, University of Santiago de Compostela
Santiago de Compostela
Galicia
ES
userId: http://scholar.google.com/citations?user=cAVkOWEAAAAJ&hl
Marcus A. González
originator
position: Full Professor
Department Zoología, Antropología Física & Genética, Faculty of Biology, University of Santiago de Compostela
Santiago de Compostela
Galicia
ES
userId: http://scholar.google.com/citations?user=NUmlPq8AAAAJ
Martin Corley
originator
position: Researcher
Pucketty Farm Cottage
Faringdon
SN7 8JP
Oxfordshire
GB
email: martin.corley@btinternet.com
userId: http://orcid.org/0000-0003-4240-8007
José Manuel Grosso-Silva
originator
position: Entomology Curator
Museu de História Natural e da Ciência da Universidade do Porto
Porto
Porto
PT
email: jmgrossosilva@gmail.com
userId: http://orcid.org/0000-0002-1094-0771
Lorenzo Quaglietta
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: lorenzo.quaglietta@cibio.up.pt
userId: http://orcid.org/0000-0002-9703-0171
Pedro Sousa
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656
Pedro Beja
originator
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Luis Martín
metadata author
position: Post-Doc Researcher
Department Zoología, Antropología Física & Genética, Faculty of Biology, University of Santiago de Compostela
Santiago de Compostela
Galicia
ES
userId: https://www.linkedin.com/profile/view?id=luismartíngonzález
Sónia Ferreira
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966
Jesús Martínez
metadata author
position: Post-Doc Researcher
Department Zoología, Antropología Física & Genética, Faculty of Biology, University of Santiago de Compostela
Santiago de Compostela
Galicia
ES
userId: http://scholar.google.com/citations?user=cAVkOWEAAAAJ&hl
Marcus A. González
metadata author
position: Full Professor
Department Zoología, Antropología Física & Genética, Faculty of Biology, University of Santiago de Compostela
Santiago de Compostela
Galicia
ES
userId: http://scholar.google.com/citations?user=NUmlPq8AAAAJ
Martin Corley
metadata author
José Manuel Grosso-Silva
metadata author
position: Entomology Curator
Museu de História Natural e da Ciência da Universidade do Porto
Porto
Porto
PT
email: jmgrossosilva@gmail.com
userId: http://orcid.org/0000-0002-1094-0771
Lorenzo Quaglietta
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: lorenzo.quaglietta@cibio.up.pt
userId: http://orcid.org/0000-0002-9703-0171
Pedro Sousa
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656
Pedro Beja
metadata author
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Pedro Sousa
user
email: prsousa@gmail.com
Pedro Beja
administrative point of contact
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Sónia Ferreira
administrative point of contact
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966