Free-living nematodes of the Mar Piccolo of Taranto (Ionian Sea), 2013-2014
Citation
Franzo A (2022): Free-living nematodes of the Mar Piccolo of Taranto (Ionian Sea), 2013-2014. v1.7. National Institute of Oceanography and Applied Geophysics. Dataset/Samplingevent. https://doi.org/10.6092/80kc-y422 accessed via GBIF.org on 2024-11-09.Description
Free-living nematodes of the Mar Piccolo of Taranto (Ionian Sea, southern Italy), 2013-2014 in the framework of the Italian flagship project RITMARE. In June 2013 and April 2014, sampling was carried out at four sites by scuba divers who collected five virtually undisturbed sediment cores using polycarbonate sample tubes (12.7 cm I.D. with a sample area of 127 cm2). In laboratory, one sediment core was subsampled by means of 3 cut-off plastic syringes (2.7 cm I.D., surface area of 5.72 cm2, 0-10 cm top sediment) in order to obtain three pseudoreplicates. The nematodes (38–1000 m) were extracted from sediment subsamples by sieving each pseudoreplicate and by centrifuging the material retained by a 38 μm mesh net with Ludox HS-40 (density of 1.15–1.18 g cm−3). For each pseudoreplicate, 100 nematodes were randomly picked out using a fine pin under a stereomicroscope (Zeiss Discovery V20, magnification 40×), transferred from formalin to glycerol through a series of ethanol-glycerol solutions and finally mounted on permanent glass slides in anhydrous glycerin. Nematodes were identified under a microscope (100x magnification) at the genus level using dedicated pictorial and identification keys.Sampling Description
Study Extent
Two monitoring campaigns were performed (June 2013 and April 2014)Sampling
Sampling was carried out at four sites by scuba diversQuality Control
Replicates were sampled and analysed to take into account biological variability.Method steps
- Sampling was carried out at four sites by scuba divers who collected five virtually undisturbed sediment cores using KC Haps bottom corer with polycarbonate sample tubes (12.7 cm I.D. with a sample area of 127 cm2). In laboratory, one sediment core was subsampled by means of 3 cut-off plastic syringes (2.7 cm I.D., surface area of 5.72 cm2, 0-10 cm top sediment) in order to obtain three pseudoreplicates. The nematodes (38–1000 μm) were extracted from sediment subsamples by sieving each pseudoreplicate and by centrifuging the material retained by a 38 μm mesh net with Ludox HS-40 (density of 1.15–1.18 g cm−3). For each pseudoreplicate, 100 nematodes were randomly picked out using a fine pin under a stereomicroscope (Zeiss Discovery V20, magnification 40×), transferred from formalin to glycerol through a series of ethanol-glycerol solutions and finally mounted on permanent glass slides in anhydrous glycerin. Nematodes were identified under a microscope (100x magnification) at the genus level using dedicated pictorial and identification keys. All data are converted to by multiplying them by a conversion factor (1.66).
Taxonomic Coverages
Free-living nematodes
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Nematodacommon name: Nematoda rank: phylum
Geographic Coverages
Bibliographic Citations
Contacts
Annalisa Franzooriginator
position: Scientist
Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
Borgo Grotta Gigante 42/C
Trieste
34010
Trieste
IT
email: afranzo@ogs.it
userId: http://orcid.org/0000-0002-4127-1558
Marina Lipizer
metadata author
position: Scientist
Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
Borgo Grotta Gigante 42/C
Trieste
34010
TS
IT
Telephone: +39 0402140310
email: mlipizer@ogs.it
userId: http://orcid.org/0000-0001-5707-6338
Paola Del Negro
originator
position: Senior Scientist
Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
Borgo Grotta Gigante 42/C
Trieste
34010
TS
IT
email: pdelnegro@ogs.it
userId: http://orcid.org/0000-0003-2465-4896
Annalisa Franzo
originator
position: Scientist
Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
Borgo Grotta Gigante, 42/C
Trieste
34010
Trieste
IT
email: afranzo@ogs.it
userId: http://orcid.org/0000-0002-4127-1558
Annalisa Franzo
administrative point of contact
position: Scientist
Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
Borgo Grotta Gigante 42/C
Trieste
34010
Trieste
IT
email: afranzo@ogs.it
userId: http://orcid.org/0000-0002-4127-1558