Tyne River Macroinvertebrates 2020
Citation
Newcastle University (2021). Tyne River Macroinvertebrates 2020. Occurrence dataset https://doi.org/10.15468/gerjpg accessed via GBIF.org on 2023-02-05.Description
Macroinvertebrates collected by kick sample from the River Tyne at NZ 107 645 on 6 November 2020 by John Bythell Sequence data to be submitted to BOLD.Purpose
Opportunistic sampling to research molecular barcoding applications.Sampling Description
Quality Control
Submissions here only include samples that were reliably identified to species level by the molecular barcode (>99% identification reliability on BOLD https://v4.boldsystems.org/) Molecular ID was only deemed reliable if consistent with UKSI-listed species.Method steps
- Representative individuals of a combined kick-sample of invertebrates were sorted, photographed and individually dissected to collect tissue samples for molecular barcoding using cytochrome oxidase subunit 1 (CO1) gene amplification using standard Folmer primers (Folmer et al 1994 Mol Mar Biol Biotechnol. 3(5):294-9) and BF2/BR2 primers ( Elbrecht & Leese (2017) Front. Environ. Sci., https://doi.org/10.3389/fenvs.2017.00011). Sequencing was carried out in both directions to create consensus assembly sequence up to 658 bp.
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
Contacts
originatorNewcastle University
Newcastle upon Tyne
NE1 7RU
Tyne and Wear
GB
homepage: http://www.ncl.ac.uk
metadata author
Newcastle University
Newcastle upon Tyne
NE1 7RU
Tyne and Wear
GB
homepage: http://www.ncl.ac.uk
distributor
NBN Atlas
Unit F, 14 - 18 St Mary's Gate, Lace Market
Nottingham
NG1 1PF
Nottinghamshire
GB
email: admin@nbnatlas.org
John Bythell
administrative point of contact
position: editor
email: john.bythell@ncl.ac.uk