eDNA‑based detection of the invasive crayfish Pacifastacus leniusculus in streams with a LAMP assay using dependent replicates to gain higher sensitivity
Citation
Porco D, Braun P (2022). eDNA‑based detection of the invasive crayfish Pacifastacus leniusculus in streams with a LAMP assay using dependent replicates to gain higher sensitivity. Version 1.10. National Museum of Natural History, Luxembourg. Occurrence dataset https://doi.org/10.15468/ek5ymk accessed via GBIF.org on 2024-12-10.Description
The records in this dataset were collected and evaluated for the following publication: Porco, D., Hermant, S., Purnomo, C.A. et al. eDNA-based detection of the invasive crayfish Pacifastacus leniusculus in streams with a LAMP assay using dependent replicates to gain higher sensitivity. Sci Rep 12, 6553 (2022). https://doi.org/10.1038/s41598-022-10545-w
Abstract of the publication: LAMP assays are becoming increasingly popular in the feld of invasive species detection but are still underused in eDNA-based monitoring. Here, we propose a LAMP assay designed to detect the North American crayfsh species Pacifastacus leniusculus in water samples from streams. The presence of P. leniusculus was detected through this new LAMP assay in all but one of the nine sites sampled. No correlation was found between ddPCR absolute concentration measurements and the number of LAMP-positive technical replicates. However, we showed that using dependent technical replicates could signifcantly enhance the detection sensitivity of the LAMP assay. Applied to other assays, it could improve sensitivity and thus allow for a more efcient use of eDNA-based LAMP assays for invasive species detection in aquatic ecosystems.
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Contacts
David Porcooriginator
position: researcher
National Museum of Natural History, Luxembourg
25 Rue Münster
Luxembourg
L-2160
LU
userId: https://orcid.org/0000-0003-1914-1973
Paul Braun
metadata author
position: Digital Curator
National Museum of Natural History, Luxembourg
25 Rue Münster
Luxembourg
L-2160
LU
email: paul.braun@mnhn.lu
userId: https://orcid.org/0000-0002-3620-6188
Paul Braun
metadata author
position: Digital Curator
National Museum of Natural History, Luxembourg
25 Rue Münster
Luxembourg
L-2160
LU
email: paul.braun@mnhn.lu
userId: https://orcid.org/0000-0002-3620-6188
David Porco
originator
position: researcher
National Museum of Natural History, Luxembourg
25 Rue Münster
Luxembourg
L-2160
LU
userId: https://orcid.org/0000-0003-1914-1973
Paul Braun
administrative point of contact
position: Digital Curator
National Museum of Natural History, Luxembourg
25 Rue Münster
Luxembourg
L-2160
LU
email: paul.braun@mnhn.lu
userId: https://orcid.org/0000-0002-3620-6188
David Porco
administrative point of contact
position: researcher
National Museum of Natural History, Luxembourg
25 Rue Münster
Luxembourg
L-2160
LU
userId: https://orcid.org/0000-0003-1914-1973