Amplicon sequencing of Tara Oceans DNA samples corresponding to size fractions for protists.

doi:10.15468/2hv1be

Amplicon sequencing of Tara Oceans DNA samples corresponding to size fractions for protists.

Citation

MGnify (2022). Amplicon sequencing of Tara Oceans DNA samples corresponding to size fractions for protists.. Sampling event dataset https://doi.org/10.15468/2hv1be accessed via GBIF.org on 2022-12-02.

Description

Analysis of 18S DNA in Tara Oceans Protists size fractions through amplicon sequencing: Seawater was filtered from different depths to retain small and large cell sizes. The DNA was extracted and amplified by PCR.

Sampling Description

Sampling

Analysis of 18S DNA in Tara Oceans Protists size fractions through amplicon sequencing: Seawater was filtered from different depths to retain small and large cell sizes. The DNA was extracted and amplified by PCR.

Method steps

Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1

Taxonomic Coverages

Geographic Coverages

Bibliographic Citations

Ibarbalz FM, Henry N, Brandão MC, Martini S, Busseni G, Byrne H, Coelho LP, Endo H, Gasol JM, Gregory AC, Mahé F, Rigonato J, Royo-Llonch M, Salazar G, Sanz-Sáez I, Scalco E, Soviadan D, Zayed AA, Zingone A, Labadie K, Ferland J, Marec C, Kandels S, Picheral M, Dimier C, Poulain J, Pisarev S, Carmichael M, Pesant S, Tara Oceans Coordinators, Babin M, Boss E, Iudicone D, Jaillon O, Acinas SG, Ogata H, Pelletier E, Stemmann L, Sullivan MB, Sunagawa S, Bopp L, de Vargas C, Karp-Boss L, Wincker P, Lombard F, Bowler C, Zinger L. 2019. Global Trends in Marine Plankton Diversity across Kingdoms of Life. Cell vol. 179 - DOI:10.1016/j.cell.2019.10.008
Faure E, Not F, Benoiston AS, Labadie K, Bittner L, Ayata SD. 2019. Mixotrophic protists display contrasted biogeographies in the global ocean. ISME J vol. 13 - DOI:10.1038/s41396-018-0340-5
Vincent FJ, Colin S, Romac S, Scalco E, Bittner L, Garcia Y, Lopes RM, Dolan JR, Zingone A, de Vargas C, Bowler C. 2018. The epibiotic life of the cosmopolitan diatom Fragilariopsis doliolus on heterotrophic ciliates in the open ocean. ISME J vol. 12 - DOI:10.1038/s41396-017-0029-1
Biard T, Bigeard E, Audic S, Poulain J, Gutierrez-Rodriguez A, Pesant S, Stemmann L, Not F. 2017. Biogeography and diversity of Collodaria (Radiolaria) in the global ocean. ISME J vol. 11 - DOI:10.1038/ismej.2017.12
Guidi L, Chaffron S, Bittner L, Eveillard D, Larhlimi A, Roux S, Darzi Y, Audic S, Berline L, Brum J, Coelho LP, Espinoza JCI, Malviya S, Sunagawa S, Dimier C, Kandels-Lewis S, Picheral M, Poulain J, Searson S, Tara Oceans coordinators, Stemmann L, Not F, Hingamp P, Speich S, Follows M, Karp-Boss L, Boss E, Ogata H, Pesant S, Weissenbach J, Wincker P, Acinas SG, Bork P, de Vargas C, Iudicone D, Sullivan MB, Raes J, Karsenti E, Bowler C, Gorsky G. 2016. Plankton networks driving carbon export in the oligotrophic ocean. Nature vol. 532 - DOI:10.1038/nature16942

Contacts

originator
The French National Sequencing Center (Genoscope)
Le Ponant Building D - 25 rue Leblanc
PARIS - RCS B 775 685 019
75015
FR
Telephone: +33 (0) 1 64 50 20 59

metadata author
The French National Sequencing Center (Genoscope)
Le Ponant Building D - 25 rue Leblanc
PARIS - RCS B 775 685 019
75015
FR
Telephone: +33 (0) 1 64 50 20 59

administrative point of contact
The French National Sequencing Center (Genoscope)
Le Ponant Building D - 25 rue Leblanc
PARIS - RCS B 775 685 019
75015
FR
Telephone: +33 (0) 1 64 50 20 59