Metabarcoding surveys of the Arctic marine environment

doi:10.15468/omitam

Metabarcoding surveys of the Arctic marine environment

Citation

MGnify (2020). Metabarcoding surveys of the Arctic marine environment. Sampling event dataset https://doi.org/10.15468/omitam accessed via GBIF.org on 2024-12-14.

Description

This is a collection of samples taken for metabarcoding of environmental samples following the Earth Microbiome Project protocol.For bacteria and archaea, we used the following primer set published by Apprill et al. (doi:10.3354/ame01753)515F GTGCCAGCMGCCGCGGTAA806RB GGACTACNVGGGTWTCTAATFor Eukaryotes, we used the following primer set published by Stoeck et al. (doi:10.1111/j.1365-294X.2009.04480.x)TAReuk454FWD1 CCAGCASCYGCGGTAATTCCTAReukREV3_modified ACTTTCGTTCTTGATYRATGA

Sampling Description

Sampling

This is a collection of samples taken for metabarcoding of environmental samples following the Earth Microbiome Project protocol.For bacteria and archaea, we used the following primer set published by Apprill et al. (doi:10.3354/ame01753)515F GTGCCAGCMGCCGCGGTAA806RB GGACTACNVGGGTWTCTAATFor Eukaryotes, we used the following primer set published by Stoeck et al. (doi:10.1111/j.1365-294X.2009.04480.x)TAReuk454FWD1 CCAGCASCYGCGGTAATTCCTAReukREV3_modified ACTTTCGTTCTTGATYRATGA

Method steps

Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1

Taxonomic Coverages

Geographic Coverages

Bibliographic Citations

Contacts

originator
University of Alaska Fairbanks

metadata author
University of Alaska Fairbanks

administrative point of contact
University of Alaska Fairbanks