Metabarcoding surveys of the Arctic marine environment
Citation
MGnify (2020). Metabarcoding surveys of the Arctic marine environment. Sampling event dataset https://doi.org/10.15468/omitam accessed via GBIF.org on 2024-10-14.Description
This is a collection of samples taken for metabarcoding of environmental samples following the Earth Microbiome Project protocol.For bacteria and archaea, we used the following primer set published by Apprill et al. (doi:10.3354/ame01753)515F GTGCCAGCMGCCGCGGTAA806RB GGACTACNVGGGTWTCTAATFor Eukaryotes, we used the following primer set published by Stoeck et al. (doi:10.1111/j.1365-294X.2009.04480.x)TAReuk454FWD1 CCAGCASCYGCGGTAATTCCTAReukREV3_modified ACTTTCGTTCTTGATYRATGASampling Description
Sampling
This is a collection of samples taken for metabarcoding of environmental samples following the Earth Microbiome Project protocol.For bacteria and archaea, we used the following primer set published by Apprill et al. (doi:10.3354/ame01753)515F GTGCCAGCMGCCGCGGTAA806RB GGACTACNVGGGTWTCTAATFor Eukaryotes, we used the following primer set published by Stoeck et al. (doi:10.1111/j.1365-294X.2009.04480.x)TAReuk454FWD1 CCAGCASCYGCGGTAATTCCTAReukREV3_modified ACTTTCGTTCTTGATYRATGAMethod steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
Contacts
originatorUniversity of Alaska Fairbanks
metadata author
University of Alaska Fairbanks
administrative point of contact
University of Alaska Fairbanks