Black Flies (Diptera: Simuliidae) of South East Asia

Study Extent

All black fly samples were collected from flowing streams.

Sampling

Black fly samples collection was done at the accessible streams and rivers, whereby these are the breeding habitats for larvae and pupae of black flies. Pupae that attached on substrates such as leaves, stems, twigs, grasses and roots were collected using forceps. Then it will be kept alive individually in vials until adult emergence before being preserved in 80% ethanol for the identification process. The methods of collection and identification followed those of Takaoka (2003) and Adler et al. (2004).

Method steps

1. The study procedures are as follows: sample collection, sample identification, sample DNA extraction, PCR.

The black fly samples were collected from different localities in Indonesia, Malaysia, Thailand and Vietnam.