FBIP: Establishment of a fully data-based and barcoded collection of mushroom pathogens for South Africa

Study Extent

Global (Australia, Belgium, Canada, Denmark, France, Germany, Ireland, Ireland, Netherlands, New Zealand, Poland, RSA, The Netherlands, UK, USA)

Sampling

A comparison between the databases of the UP mushroom research group and PPRI concerning mushroom pathogens was done to identify any isolates that were not yet contained in both collections; these were revived and exchanged. Missing collection data were added as far as possible. The UP database was upgraded by an MSc student who also coordinated plating of cultures for barcoding and addition to the PPRI collection. Each isolate will have data fields containing the following information: o Numbers: PPRI number, UP collection number, any other collection/reference numbers (e.g. in case of foreign specimens) o Identity: genus, species, authors, synonyms o Higher classification: family, order, class etc. o Dates: dates collected, isolated, preserved, deposited o Host / substrate information: host type (= fungus), scientific and common name o Locality details: country, province, town, farm o Collector name, identified by, verified by o Barcoded: Y / N, and barcode reference no. referring to sequence file – to be added For barcoding, the mushroom pathogens (per genus) of the UP collection were plated and checked for viability and purity. This involved plating on required growth media, incubation, checking viability and purity and preservation of voucher cultures under water, under oil, ultra-low freezing as well as freeze drying. The SA isolates were done first, followed by the foreign ones.

Quality Control

None

Method steps

1. NA

Identification of mushroom pathogens

1. Fungi
   rank: kingdom

Global (Australia, Belgium, Canada, Denmark, France, Germany, Ireland, Ireland, Netherlands, New Zealand, Poland, RSA, The Netherlands, UK, USA)