Evaluation of filtration and DNA extraction methods for environmental DNA biodiversity assessments across multiple trophic levels.
Citation
MGnify (2019). Evaluation of filtration and DNA extraction methods for environmental DNA biodiversity assessments across multiple trophic levels.. Sampling event dataset https://doi.org/10.15468/zaiagx accessed via GBIF.org on 2021-12-02.Description
Methods evaluation for sample collection and nucleic acid extraction from multiple trophic levels simultaneously (i.e. bacteria, phytoplankton, zooplankton, vertebrates). Specifically, filter types and DNA extraction kits were evaluated for producing Illumina MiSeq reads for 12S, 16S and 18S amplicons from water samples collected in Monterey Bay California.Sampling Description
Sampling
Methods evaluation for sample collection and nucleic acid extraction from multiple trophic levels simultaneously (i.e. bacteria, phytoplankton, zooplankton, vertebrates). Specifically, filter types and DNA extraction kits were evaluated for producing Illumina MiSeq reads for 12S, 16S and 18S amplicons from water samples collected in Monterey Bay California.Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
Contacts
originatorMonterey Bay Aquarium Research Institution
metadata author
Monterey Bay Aquarium Research Institution
administrative point of contact
Monterey Bay Aquarium Research Institution