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Evaluation of filtration and DNA extraction methods for environmental DNA biodiversity assessments across multiple trophic levels.

Dataset homepage

Citation

MGnify (2019). Evaluation of filtration and DNA extraction methods for environmental DNA biodiversity assessments across multiple trophic levels… Sampling event dataset https://doi.org/10.15468/zaiagx accessed via GBIF.org on 2019-12-16.

Description

Methods evaluation for sample collection and nucleic acid extraction from multiple trophic levels simultaneously (i.e. bacteria, phytoplankton, zooplankton, vertebrates). Specifically, filter types and DNA extraction kits were evaluated for producing Illumina MiSeq reads for 12S, 16S and 18S amplicons from water samples collected in Monterey Bay California.

Sampling Description

Sampling

Methods evaluation for sample collection and nucleic acid extraction from multiple trophic levels simultaneously (i.e. bacteria, phytoplankton, zooplankton, vertebrates). Specifically, filter types and DNA extraction kits were evaluated for producing Illumina MiSeq reads for 12S, 16S and 18S amplicons from water samples collected in Monterey Bay California.

Method steps

  1. Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1

Taxonomic Coverages

Geographic Coverages

Bibliographic Citations

Contacts

originator
BioProject
metadata author
BioProject
administrative point of contact
BioProject
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