Actualización de la Colección de Microorganismos de importancia acuática

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La colección CAIM ya gozó de un proyecto de computarización por parte de la CONABIO (CC007). Durante este proyecto se computarizó la Colección, pero varias de las cepas (261) quedaron incompletas en su identificación. De las 1813 cepas (registros) faltan por identificar 210 (11.6 %) y otros 51 sólo están a nivel de género; además se han adquirido varios nuevos ejemplares. Para poder llevar a cabo la actualización de la base de datos (BIOTICA), uno de los objetivos que no se pudo satisfacer plenamente en la propuesta anterior, es necesario identificar esas cepas. Para cumplir con este objetivo, en la presenta propuesta se realizarán secuenciaciones de genes útiles ("housekeeping") para poder llegar a una identificación correcta de las cepas bacterianas faltantes. Debido a que éstos genes ya se encuentran secuenciados y disponibles en bases de datos públicas (GenBank), los resultados que se ajusten con una similitud muy elevada (superior al 97-98% para el caso del 16S rARN, por ejemplo), será el criterio para identificar la cepa como perteneciente a esa especie. En caso de que el índice de similitud sea menor, se catalogará como potencial nueva especie y sujeta a posterior análisis. Con estos datos, se actualizará la base de datos para reflejar estas nuevas determinaciones y adicionar las bacterias que se han adquirido recientemente.

Reino: 1 Filo: 5 Clase: 4 Orden: 14 Familia: 22 Género: 39 Especie: 121 Epitetoinfraespecifico: 4

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Bibliographic Citations

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