Exploring Fronts With Multiple Robots Schmidt Ocean Institute Cruise to the Pacific Ocean in 2018
Citation
MGnify (2019). Exploring Fronts With Multiple Robots Schmidt Ocean Institute Cruise to the Pacific Ocean in 2018. Sampling event dataset https://doi.org/10.15468/99a81z accessed via GBIF.org on 2023-05-29.Description
Samples were obtained during the Schmidt Ocean Institute cruise "Exploring Fronts With Mutiple Robots" to the Pacific Ocean in 2018 (https://schmidtocean.org/cruise/exploring_fronts_with_multiple_aerial-surface-underwater-vehicles/). Water samples for microplankton analysis were collected using a multi water sampler (rosette) from the research vessel "Falkor" and concentrated on board in a 0.2 m Sterivex filter. This expedition target the Subtropical Front, located approximately 1,000 nautical miles off the coast of Southern California. The study includes samples from: (i) amplicon sequencing after 18S amplification by PCR using TAReuk454FWD1/ TAReukREV3_modified primer set and (ii) amplicon sequencing after 16S amplification by PCR using 515F/926R primer set. Libraries were constructed according to Illumina Library protocol.Sampling Description
Sampling
Samples were obtained during the Schmidt Ocean Institute cruise "Exploring Fronts With Mutiple Robots" to the Pacific Ocean in 2018 (https://schmidtocean.org/cruise/exploring_fronts_with_multiple_aerial-surface-underwater-vehicles/). Water samples for microplankton analysis were collected using a multi water sampler (rosette) from the research vessel "Falkor" and concentrated on board in a 0.2 m Sterivex filter. This expedition target the Subtropical Front, located approximately 1,000 nautical miles off the coast of Southern California. The study includes samples from: (i) amplicon sequencing after 18S amplification by PCR using TAReuk454FWD1/ TAReukREV3_modified primer set and (ii) amplicon sequencing after 16S amplification by PCR using 515F/926R primer set. Libraries were constructed according to Illumina Library protocol.Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
- Semedo M, Lopes E, Baptista MS, Oller-Ruiz A, Gilabert J, Tomasino MP, Magalhães C. 2021. Depth Profile of Nitrifying Archaeal and Bacterial Communities in the Remote Oligotrophic Waters of the North Pacific. Front Microbiol vol. 12 - DOI:10.3389/fmicb.2021.624071
Contacts
originatorCIIMAR - Interdisciplinary Centre of Marine and Environmental Research
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CIIMAR - Interdisciplinary Centre of Marine and Environmental Research
administrative point of contact
CIIMAR - Interdisciplinary Centre of Marine and Environmental Research