Diversity of bacterioneuston and bacterioplankton in coastal waters of Misaki, Japan

Sampling

Deep sequencing of environmental DNA from surface microlayer obtained using polycarbonate membrane (depth= 33 micrometer), glass plate (depth=42 micrometer) and drum sampler (depth=35 micrometer). The bacterial communities obtained using these three surface microlayer samplers were compared to control underlying water obtained from the depth of 20 cm.

Method steps

1. Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1