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Response of soil bacteria to anthropogenic soil variables at large spatial scales

Dataset homepage

Citation

MGnify (2020). Response of soil bacteria to anthropogenic soil variables at large spatial scales. Sampling event dataset https://doi.org/10.15468/goblzl accessed via GBIF.org on 2022-07-07.

Description

We sought to determine the relative influences of spatial and environmental factors on entire bacterial communities and individual taxa, in both natural and human-impacted soils in the upper North Island of New Zealand. We conducted 16S rRNA (v3-v4 region) amplicon sequencing on soil samples from 110 native forests, plantation forest, horticultural or pastoral sites, which constitute the dominant land uses within temperate climate zones. These sites were located between 5 m and 300 km apart. This bacterial data was paired with extensive site metadata to determine which environmental variables were correlated with changes in bacterial community composition as a whole, as well as with changes in the abundance of specific taxa.

Sampling Description

Sampling

We sought to determine the relative influences of spatial and environmental factors on entire bacterial communities and individual taxa, in both natural and human-impacted soils in the upper North Island of New Zealand. We conducted 16S rRNA (v3-v4 region) amplicon sequencing on soil samples from 110 native forests, plantation forest, horticultural or pastoral sites, which constitute the dominant land uses within temperate climate zones. These sites were located between 5 m and 300 km apart. This bacterial data was paired with extensive site metadata to determine which environmental variables were correlated with changes in bacterial community composition as a whole, as well as with changes in the abundance of specific taxa.

Method steps

  1. Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1

Taxonomic Coverages

Geographic Coverages

Bibliographic Citations

Contacts

originator
The University of Auckland
metadata author
The University of Auckland
administrative point of contact
The University of Auckland
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