Microbial metabarcoding surveys (Bacteria, Archaea and Eukaryota) of the arctic marine environment

Eric Collins

doi:10.15468/mx3u6c

Microbial metabarcoding surveys (Bacteria, Archaea and Eukaryota) of the arctic marine environment

Citation

Collins E, Sweetlove M (2019). Microbial metabarcoding surveys (Bacteria, Archaea and Eukaryota) of the arctic marine environment. SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/mx3u6c accessed via GBIF.org on 2020-01-21.

Description

Large amplicon sequencing dataset (Illumina MiSeq) targeting Bacteria/Archaea (16S ssu rRNA gene) and Eukaryota (18S ssu rRNA gene) from samples taken in the Arctic marine environment, following the Earth Microbiome Project protocol.

Taxonomic Coverages

Eukaryotes in marine environments, profiled by sequencing the v4 region of the 18S ssu rRNA gene, using the primer set published by Stoeck et al. (doi:10.1111/j.1365-294X.2009.04480.x) TAReuk454FWD1 CCAGCASCYGCGGTAATTCC TAReukREV3_modified ACTTTCGTTCTTGATYRATGA

Eukaryota
common name: Eukaryotes rank: domain

Bacteria and Archaea in marine environments, profiled by sequencing the v4 region of the 16S ssu rRNA gene, using the primer set published by Apprill et al. (doi:10.3354/ame01753) 515F GTGCCAGCMGCCGCGGTAA 806RB GGACTACNVGGGTWTCTAAT

Bacteria
common name: Bacteria rank: domain
Archaea
common name: Archaea rank: domain

Geographic Coverages

Complete coverage of the Arctic marine environments.

Bibliographic Citations

Contacts

Eric Collins
originator
position: Assistant professor
College of Fisheries and Ocean Sciences
Fairbanks
US
email: recollins@alaska.edu

Maxime Sweetlove
metadata author
position: Research assistant
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
Brussels
1000
BE
email: msweetlove@naturalsciences.be

Eric Collins
administrative point of contact
position: Assistant professor
College of Fisheries and Ocean Sciences
Fairbanks
US
email: recollins@alaska.edu