Microbial metabarcoding surveys (Bacteria, Archaea and Eukaryota) of the arctic marine environment
Citation
Collins E, Sweetlove M (2019). Microbial metabarcoding surveys (Bacteria, Archaea and Eukaryota) of the arctic marine environment. SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/mx3u6c accessed via GBIF.org on 2021-01-24.Description
Large amplicon sequencing dataset (Illumina MiSeq) targeting Bacteria/Archaea (16S ssu rRNA gene) and Eukaryota (18S ssu rRNA gene) from samples taken in the Arctic marine environment, following the Earth Microbiome Project protocol.Taxonomic Coverages
Eukaryotes in marine environments, profiled by sequencing the v4 region of the 18S ssu rRNA gene, using the primer set published by Stoeck et al. (doi:10.1111/j.1365-294X.2009.04480.x)
TAReuk454FWD1 CCAGCASCYGCGGTAATTCC
TAReukREV3_modified ACTTTCGTTCTTGATYRATGA
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Eukaryotacommon name: Eukaryotes rank: domain
Bacteria and Archaea in marine environments, profiled by sequencing the v4 region of the 16S ssu rRNA gene, using the primer set published by Apprill et al. (doi:10.3354/ame01753)
515F GTGCCAGCMGCCGCGGTAA
806RB GGACTACNVGGGTWTCTAAT
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Bacteriacommon name: Bacteria rank: domain
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Archaeacommon name: Archaea rank: domain
Geographic Coverages
Complete coverage of the Arctic marine environments.
Bibliographic Citations
Contacts
Eric Collinsoriginator
position: Assistant professor
College of Fisheries and Ocean Sciences
Fairbanks
US
email: recollins@alaska.edu
Maxime Sweetlove
metadata author
position: Research assistant
Royal Belgian Institute of Natural Sciences
Rue Vautier 29
Brussels
1000
BE
email: msweetlove@naturalsciences.be
Eric Collins
administrative point of contact
position: Assistant professor
College of Fisheries and Ocean Sciences
Fairbanks
US
email: recollins@alaska.edu