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Quantitative monitoring of environmental DNA using high-throughput sequencing

Dataset homepage

Citation

MGnify (2019). Quantitative monitoring of environmental DNA using high-throughput sequencing. Sampling event dataset https://doi.org/10.15468/bhj7px accessed via GBIF.org on 2023-02-03.

Description

The goal of this project is to monitor the dynamics of environmental DNA (e.g., fish DNA found in a water sample) quantitatively. By adding internal standard DNA, a standard curve (i.e., the relationship between the number of DNA copy and sequence read) will be drawn. Based on the standard curve, sequence reads of unknown DNA (non-standard DNA) will be converted to the number of DNA copy. The environmental DNA metabarcoding combined with internal standard DNA will enable quantitative monitoring of multispecies environmental DNA.

Sampling Description

Sampling

The goal of this project is to monitor the dynamics of environmental DNA (e.g., fish DNA found in a water sample) quantitatively. By adding internal standard DNA, a standard curve (i.e., the relationship between the number of DNA copy and sequence read) will be drawn. Based on the standard curve, sequence reads of unknown DNA (non-standard DNA) will be converted to the number of DNA copy. The environmental DNA metabarcoding combined with internal standard DNA will enable quantitative monitoring of multispecies environmental DNA.

Method steps

  1. Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1

Taxonomic Coverages

Geographic Coverages

Bibliographic Citations

Contacts

originator
RYUKOKU
metadata author
RYUKOKU
administrative point of contact
RYUKOKU
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