Quantitative monitoring of environmental DNA using high-throughput sequencing

Sampling

The goal of this project is to monitor the dynamics of environmental DNA (e.g., fish DNA found in a water sample) quantitatively. By adding internal standard DNA, a standard curve (i.e., the relationship between the number of DNA copy and sequence read) will be drawn. Based on the standard curve, sequence reads of unknown DNA (non-standard DNA) will be converted to the number of DNA copy. The environmental DNA metabarcoding combined with internal standard DNA will enable quantitative monitoring of multispecies environmental DNA.

Method steps

1. Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1