16S sequencing of environmental samples to test the impact of sediment source

Sampling

The Broad Institute sequenced environmental samples to tests the impact of sediment source, supplemental cellulose source, and depth within Winogradsky columns, on microbial community structure. The study was conducted, in part, within introductory microbiology courses at Vassar College (Poughkeepsie, NY) and Williams College (Williamstown, MA). Sequencing was conducted at the Broad Institute (Cambridge, MA). Winogradsky columns were created from sediment collected near the edge of two small ponds (under approximately 15-30 cm of water) in late October 2008. Ponds were located near the campus of Williams College in Williamstown, Massachusetts: Buxton Pond (N 42.70o (42 o 42 15) W 73.2114 o (73 o 12 41) and Ephs Pond (N 42.7201o (42 o 43 12) W 73.1975 o (73 o 11 51).

Method steps

1. Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1