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16S sequencing of environmental samples to test the impact of sediment source

Dataset homepage

Citation

MGnify (2019). 16S sequencing of environmental samples to test the impact of sediment source. Sampling event dataset https://doi.org/10.15468/xqn0tq accessed via GBIF.org on 2023-03-29.

Description

The Broad Institute sequenced environmental samples to tests the impact of sediment source, supplemental cellulose source, and depth within Winogradsky columns, on microbial community structure. The study was conducted, in part, within introductory microbiology courses at Vassar College (Poughkeepsie, NY) and Williams College (Williamstown, MA). Sequencing was conducted at the Broad Institute (Cambridge, MA). Winogradsky columns were created from sediment collected near the edge of two small ponds (under approximately 15-30 cm of water) in late October 2008. Ponds were located near the campus of Williams College in Williamstown, Massachusetts: Buxton Pond (N 42.70o (42 o 42 15) W 73.2114 o (73 o 12 41) and Ephs Pond (N 42.7201o (42 o 43 12) W 73.1975 o (73 o 11 51).

Sampling Description

Sampling

The Broad Institute sequenced environmental samples to tests the impact of sediment source, supplemental cellulose source, and depth within Winogradsky columns, on microbial community structure. The study was conducted, in part, within introductory microbiology courses at Vassar College (Poughkeepsie, NY) and Williams College (Williamstown, MA). Sequencing was conducted at the Broad Institute (Cambridge, MA). Winogradsky columns were created from sediment collected near the edge of two small ponds (under approximately 15-30 cm of water) in late October 2008. Ponds were located near the campus of Williams College in Williamstown, Massachusetts: Buxton Pond (N 42.70o (42 o 42 15) W 73.2114 o (73 o 12 41) and Ephs Pond (N 42.7201o (42 o 43 12) W 73.1975 o (73 o 11 51).

Method steps

  1. Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1

Taxonomic Coverages

Geographic Coverages

Bibliographic Citations

  1. Rundell EA, Banta LM, Ward DV, Watts CD, Birren B, Esteban DJ. 2014. 16S rRNA gene survey of microbial communities in Winogradsky columns. PLoS One vol. 9 - DOI:10.1371/journal.pone.0104134

Contacts

originator
Broad Institute
metadata author
Broad Institute
administrative point of contact
Broad Institute
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