Microorganismos asociados con la filósfera de plantas de Espeletia en el PNN los Nevados

Study Extent

Microbial isolates were obtained from Espeletia leaves sampled from plants located in páramo ecosystems (04°52'27'' N 75°15'51.4'' W).

Sampling

Leaf samples from various plant parts were taken, in triplicate, and transported to the lab where they were processed to recover culturable microorganisms and DNA.

Method steps

1. Microbes were recovered from leaf surfaces by plating on solid R2A Agar (Difco) and Tryptone soy agar (TSA, Oxoid), and incubating several days at 25ºC. Strains were purified by re-streaking, characterized by growth on various media, and checked for the ability to withstand UVC and temperature changes. Strains were identified by sequence analysis of either the 16S rRNA gene or the ITS region.

Microorganismos pertencientes a Bacteria y Eukarya, en la mayoría a nivel de género.

1. Bacteria
   rank: domain
2. Eukarya
   rank: domain

The samples were collected at a specific location within the PNN los Nevados (04°52'27'' N 75°15'51.4'' W). This park is located on the Cordillera Central in Colombia, it has altitudes between 2,600 and 5,321 m.a.s.l., and an area of approximately 58,300 hectares with diverse ecosystems (Van Wyngaarden, W., & Fandiño, M. 2002). It is located within the Departments of Caldas (Municipio de Villamaría), Risaralda (Municipios de Santa Rosa de Cabal y Pereira), Quindío (Municipio de Salento) and Tolima (Municipios de Ibagué, Anzoátegui, Santa Isabel, Murillo, Villahermosa, Casabianca y Herveo)(www.parquesnacionales.gov.co/).