Collection and observation of macrofungi from Vakh river basin (Western Siberia)

Study Extent

Random forays were undertaken in august of 2011 at 6 locations down the Wakh River starting from the Bolshoi Laryak village: 1) Bol’shoy lar’yak village vicinities, floodplain of the Big Pasol River, N61°4.8'. E80°46.8'; 2) Bol’shoy lar’yak village vicinities, N61°4.8'c. E80°47.7; 3) the left bank of the Vakh river, 10 km downstream from the village. Lar’yak, N61°11.4, E80°25.2; 4) the right bank of the Vakh river, 10 km to south-west from the Lar’yak village, N61°3.6, E80°03'; 5) the left bank of the Wakh river, 41 km to the east from the Bol’shetarkhovo village, N61°3.6., E77°54.6'; 6) the left bank of the Vakh river, Izluchinsk vicinity, N61°5.4, E77°2.4'. 150 specimens were collected.

Sampling

Collections were made, documented and preserved using the standard methods (Bondartsev, Zinger, 1955). Macroscopical descriptions were based on the study of both fresh and dried material as well as from the photographs. Dried material was examined using standard microscopical techniques. Microstructures were observed and measured at 400x and at 1000x in squash preparations in 5% KOH, Congo Red and Melzer’s reagent using a Leica light microscope. The pileipellis was examined in radial section pileus preparations.

Quality Control

Collection is stored in Surgut State University Herbarium. All determinations could be checked by the request of specimens. Autor's email is provided.

Method steps

1. Forays were undertaken randomly.
2. Specimens were photographed in situ for macromorphological features
3. Fading out features like smell and taste were described in field.
4. Sporocarps were extracted from substrate and putted into aluminum foil.
5. Sporocarps were dried in the mushroom dryer under 40-50 °C
6. Dried specimens are stored in Ziplocs and paper envelopes
7. Microstructures were observed and measured at 400x and at 1000x in squashed preparations in 5% KOH, Congo Red and Melzer’s reagent using a Leica light microscope. Pileipellis was examined in radial section preparations.
8. Microphotographs were obtained using UCMOS14000KPA.
9. Microstructures were measured using TopView software.

Studies were conducted in the middle and lower reaches of the Vakh River.