NEON Biorepository Fish Collection (DNA Extracts)
Citation
National Ecological Observatory Network (2024). NEON Biorepository Fish Collection (DNA Extracts). Occurrence dataset https://doi.org/10.15468/qtg4n6 accessed via GBIF.org on 2024-10-14.Description
This collection contains genetic extracts from select fish collected during fish sampling at aquatic sites (NEON sample class: mam_barcoding_in.dnaSubsampleID.fsh). Fish are sampled using a combination of electrofishing, gill-nets and mini-fyke nets in wadeable streams and lakes. Fin clips are collected from 5-10 individuals of a target species. Tissues are preserved in an appropriate tissue vial and shipped to an external lab. DNA is extracted and target sequences amplified via PCR. Barcodes of cytochrome oxidase are generated per specimen. Genetic extracts are stored dehydrated in 96-well plates from the Canadian Centre for DNA Barcoding are archived in the NEON Biorepository at -80 degrees Celsius. When eluted into 20 uL, concentrations of 5-30 ng/uL are expected. See related links below for protocols and NEON related data products. Information about the Canadian Centre for DNA Barcoding can be found at https://ccdb.ca/.
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
Contacts
originatorNEON Biorepository Data Portal
email: biorepo@asu.edu
homepage: https://biorepo.neonscience.org/portal/index.php
metadata author
NEON Biorepository Data Portal
email: biorepo@asu.edu
homepage: https://biorepo.neonscience.org/portal/index.php
administrative point of contact
NEON Biorepository Fish Collection (DNA Extracts)
email: biorepo@asu.edu
homepage: https://data.neonscience.org/data-products/DP1.20107.001
NEON Biorepository
content provider
email: biorepo@asu.edu
Azhar Husain
content provider
position: Collection Manager
email: azhar.husain@asu.edu