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The InBIO Barcoding Initiative Database: Hemiptera 01

Dataset homepage

Citation

Sousa P, Grosso-Silva J M, Andrade R, Beja P, Ferreira S (2021). The InBIO Barcoding Initiative Database: Hemiptera 01. Version 1.2. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/8hn25y accessed via GBIF.org on 2022-08-19.

Description

The dataset contains 135 records of Hemiptera species collected from 2015 to 2019 in continental Portugal (Sousa et al., 2021). The species represented in the dataset, 90 in total, correspond to about 7.5% of the known true bugs diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Hemiptera. Specimens were captured during fieldwork by direct search of specimens or by sweeping the vegetation and stored in 96% ethanol. All specimens were identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Purpose

The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Sampling Description

Study Extent

Specimens were collected in 60 different localities of continental Portugal. Sampling was conducted from 2015 to 2019, in several field work expeditions.

Sampling

The studied material was collected in 60 different localities from continental Portugal. Sampling was conducted between 2015 and 2019 on a wide range of habitats, both by direct search of specimens or using a hand-held sweep net. Collected specimens were examined using a binocular stereoscopic microscope and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature.

Quality Control

All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org).

Method steps

  1. Specimens were collected in 60 different localities of continental Portugal. Fieldwork was carried out between 2015 and 2019.
  2. Specimens were collected during fieldwork by direct search of specimens or by sweeping the vegetation and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
  3. Specimens were morphologically identified with the assistance of a stereoscopic microscope and using the available literature. A subset (23%) was identified using the BOLD Identification Engine directly.
  4. DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For one specimens of Ceraleptus lividus only a 418 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
  5. All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
  6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Taxonomic Coverages

This dataset is composed of data relating to 135 Hemiptera specimens. All specimens were determined to species level or below. Overall, 90 species and one subspecies are represented in the dataset. These species belong to 31 families. The Pentatomidae family accounts for 20% of the total collected specimens, and no other family accounts for more than 8%. The Pentatomidae and Miridae families combined account for 26% of the total taxa represented, and no other family accounts for more than 7%. Eleven families are represented by a single taxon and ten by two taxa.
  1. Animalia
    rank: kingdom
  2. Arthropoda
    rank: phylum
  3. Insecta
    rank: class
  4. Hemiptera
    rank: order
  5. Acanthosomatidae
    rank: family
  6. Alydidae
    rank: family
  7. Aphalaridae
    rank: family
  8. Aphrophoridae
    rank: family
  9. Aradidae
    rank: family
  10. Auchenorrhyncha
    rank: family
  11. Berytidae
    rank: family
  12. Cercopidae
    rank: family
  13. Cicadellidae
    rank: family
  14. Cicadidae
    rank: family
  15. Coreidae
    rank: family
  16. Corixidae
    rank: family
  17. Cydnidae
    rank: family
  18. Delphacidae
    rank: family
  19. Dictyopharidae
    rank: family
  20. Flatidae
    rank: family
  21. Gerridae
    rank: family
  22. Heteroptera
    rank: family
  23. Hydrometridae
    rank: family
  24. Lygaeidae
    rank: family
  25. Membracidae
    rank: family
  26. Miridae
    rank: family
  27. Nabidae
    rank: family
  28. Nepidae
    rank: family
  29. Notonectidae
    rank: family
  30. Pentatomidae
    rank: family
  31. Potamocoridae
    rank: family
  32. Pyrrhocoridae
    rank: family
  33. Reduviidae
    rank: family
  34. Rhopalidae
    rank: family
  35. Rhyparochromidae
    rank: family
  36. Stenocephalidae
    rank: family
  37. Thaumastocoridae
    rank: family

Geographic Coverages

Continental Portugal.

Bibliographic Citations

  1. Sousa, P., Grosso-Silva, J.M., Andrade, R., Chaves, C., Pinto, J., Paupério, J., Beja, P. & Ferreira, S. (2021). The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Hemiptera 01. Biodiversity Data Journal, 9: e65314. doi: 10.3897/BDJ.9.e65314 -

Contacts

Pedro Sousa
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656
José M. Grosso-Silva
originator
position: Entomology Curator
Museu de História Natural e da Ciência da Universidade do Porto
Porto
Porto
PT
email: jmgrossosilva@gmail.com
userId: http://orcid.org/0000-0002-1094-0771
Rui Andrade
originator
position: Researcher
Rua Calouste Gulbenkian, 237
Porto
4050-145
Porto
PT
email: ruiamandrade@yahoo.com
Pedro Beja
originator
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Sónia Ferreira
originator
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966
Pedro Sousa
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: psousa@cibio.up.pt
userId: http://orcid.org/0000-0002-5859-9656
José M. Grosso-Silva
metadata author
position: Entomology Curator
Museu de História Natural e da Ciência da Universidade do Porto
Porto
Porto
PT
email: jmgrossosilva@gmail.com
userId: http://orcid.org/0000-0002-1094-0771
Rui Andrade
metadata author
position: Researcher
Rua Calouste Gulbenkian, 237
Porto
4050-145
Porto
PT
email: ruiamandrade@yahoo.com
Pedro Beja
metadata author
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Sónia Ferreira
metadata author
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966
Pedro Beja
administrative point of contact
position: Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: pbeja@cibio.up.pt
userId: http://orcid.org/0000-0001-8164-0760
Sónia Ferreira
administrative point of contact
position: Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
Vairão
4485-661
Porto
PT
Telephone: +351 252660411
email: hiporame@gmail.com
userId: http://orcid.org/0000-0002-6884-3966
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