Lipid classes in zooplankton Nansen Legacy Q4
Citation
Kohlbach D, Wold A, Keck Al-Habahbeh A, Woll M, Graeve M, Hop H, Assmy P (2022). Lipid classes in zooplankton Nansen Legacy Q4. The Nansen Legacy Project. Sampling event dataset https://doi.org/10.21334/npolar.2022.d436b683 accessed via GBIF.org on 2023-05-28.Description
Relative proportions of lipid classes in pelagic zooplankton collected during Nansen Legacy seasonal cruise Q4 (28 November - 17 December 2019)Sampling Description
Study Extent
Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°NSampling
Samples of 5 zooplankton taxa including copepods and amphipods were collected at six stations (P1, P4, P5, P6, P7, NLEG3), using MIK nets (1200 μm with 500 μm cod end), Macroplankton trawl (multiple mesh sizes along the net, tapering to 8 mm at its end) and Bongo nets (64 and 180 μm). Samples were sorted into species and stage/size groups onboard the ship and immediately frozen at -80 ºC.Method steps
- Lipid classes were analysed at the Alfred Wegener Institute, Bremerhaven, Germany. All samples were freeze-dried for at least 24 h prior to lipid extraction (-45 °C, 0.1 mbar). Zooplankton were homogenized mechanically using a Potter-Elvehjem homogenizer. Total lipids were extracted using a modified procedure from Folch et al. (1957) with dichloromethane/methanol (2:1, v/v). The extracted lipids were cleaned with 0.88 % potassium chloride solution. Lipid class analysis was performed directly on the extracted lipids (Graeve and Janssen, 2009) via high performance liquid chromatography. Lipid classes were distinguished into neutral (i.e. storage) lipids and polar (i.e. membrane) lipids. Folch, J., Lees, M., and Stanley, G.H.S. (1957). A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226(1), 497-509 Graeve, M., and Janssen, D. (2009). Improved separation and quantification of neutral and polar lipid classes by HPLC–ELSD using a monolithic silica phase: application to exceptional marine lipids. J. Chromatogr. B 877(20-21), 1815-1819
Taxonomic Coverages
Copepods, amphipods
-
Calanus glacialis
-
Calanus hyperboreus
-
Calanus finmarchicus
-
Themisto abyssorum
-
Themisto libellula
Geographic Coverages
Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N
Bibliographic Citations
- Kohlbach D, Schmidt K, Hop H, Wold A, Al-Habahbeh AK, Belt ST, Woll M, Graeve M, Smik L, Atkinson A and Assmy P (2021) Winter Carnivory and Diapause Counteract the Reliance on Ice Algae by Barents Sea Zooplankton. Front. Mar. Sci. 8:640050 - doi: 10.3389/fmars.2021.640050
Contacts
Doreen Kohlbachoriginator
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no
Anette Wold
originator
position: Engineer
Norwegian Polar Institute
Tromsø
NO
email: anette.wold@npolar.no
Amalia Keck Al-Habahbeh
originator
position: PhD student
UiT The Arctic University of Norway
Tromsø
NO
email: amalia.keck@uit.no
Matthias Woll
originator
position: Engineer
Alfred Wegener Institute
Bremerhaven
DE
email: matthias.woll@awi.de
Martin Graeve
originator
position: Researcher
Alfred Wegener Institute
Bremerhaven
DE
email: martin.graeve@awi.de
Haakon Hop
originator
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: haakon.hop@awi.de
Philipp Assmy
originator
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: philipp.assmy@npolar.no
Doreen Kohlbach
metadata author
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no
Doreen Kohlbach
author
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no
Anette Wold
author
position: Engineer
Norwegian Polar Institute
Tromsø
NO
email: anette.wold@npolar.no
Amalia Keck Al-Habahbeh
author
position: PhD student
UiT The Arctic University of Norway
Tromsø
NO
email: amalia.keck@uit.no
Matthias Woll
author
position: Engineer
Alfred Wegener Institute
Bremerhaven
DE
email: matthias.woll@awi.de
Martin Graeve
author
position: Researcher
Alfred Wegener Institute
Bremerhaven
DE
email: martin.graeve@awi.de
Haakon Hop
author
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: haakon.hop@npolar.no
Philipp Assmy
author
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: philipp.assmy@npolar.no
Doreen Kohlbach
administrative point of contact
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no