The InBIO Barcoding Initiative Database: A taxonomic revision of the Western Palaearctic genus Cacochroa Heinemann, 1870 (Lepidoptera, Depressariidae)

Study Extent

Specimens were collected in ten different localities of seven different countries, most of them bordering the Mediterranean Sea. Sampling was conducted from 1988 to 2016.

Sampling

Sampling of specimens was done at ten different localities of seven different countries, Bulgaria, France, Greece, Italy and Portugal in Europe and Morocco and Tunisia in in two Africa. Sampling was conducted between 1988 to 2016. Captured specimens were preserved in 96% ethanol, with one exception kept in dry storage. Preserved specimens are deposited in two Museums: Natural History Museum, London and the Natural History Museum of Denmark, Zoological Collection, University of Copenhagen and in three research collections: Martin Corley’s, Zdenko Tokár’s and Giorgio Baldizzone’s.

Quality Control

The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Method steps

1. Sampling of specimens was done at ten different localities of seven different European and African countries., collected between 1988 and 2016.
2. The captured specimen was preserved in 96% ethanol with a single exception kept in dry storage.
3. The specimen was morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley, 2018; Corley. & Ferreira 2019; Heinemann, 1870; Herrich-Schäffer, [1853−1855] 1847−1855; Lvovsky, 1981 and 2000; Rebel, 1901).
4. DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
5. The obtained sequences were submitted to BOLD database and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

This dataset is composed of data relating to 11 Lepidoptera specimens. All specimens were determined to species level. Five species belonging to two genera, Cacochroa Heinemann, 1870 and Rosetea Corley & Ferreira 2019, are represented in the dataset. Both genera belong to the family Depressariidae.

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Insecta
   rank: class
4. Lepidoptera
   rank: order
5. Depressariidae
   rank: family

Southern Europe and North Africa