Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition
Citation
Bielewicz S, Bell E, Kong W, Froedberg I, Priscu J, Morgan-Kiss R, Sweetlove M (2021). Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/t628ps accessed via GBIF.org on 2023-06-05.Description
Amplicon sequencing dataset targeting eukaryote microorganisms (18S ssu rRNA marker gene) in permanently ice-covered lakes in the McMurdo Dry Valleys (Antarctica). Samples (n=89) were taken in the photic zone of the east lobe (ELB) and west lobe (WLB) of the chemically stratified Lake Bonney.Sampling Description
Study Extent
Lake Bonney in the McMurdo Dry Valleys (Antarctica). Samples were collected at 6, 13, 18, and 20m in the east lobe (ELB) and 10, 13, 15, and 20m in the west lobe (WLB) of Lake Bonney from February to April 2008. All collection depths are relative to the piezometric water level in the ice borehole.Sampling
Samples (1 liter) were vacuum concentrated (5 kPa) onto 47 mm 0.45 µm Durapore polyvinylidene fluoride membrane filters (Millipore). The filters were frozen immediately in liquid nitrogen and transported frozen to McMurdo Station, where they were stored at -80 degrees C until DNA extraction.Method steps
- DNA was isolated from a whole filter using the MP DNA kit (MP Biomedicals, CA) following the kit’s instruction. Sequencing was performed using the BigDye Terminator v3.1 cycle sequencing kit (ABI, CA) with M13R primer and the fragments were sequenced on an Applied Biosystems 3730×l DNA Analyzer (ABI, CA). The 18S rRNA gene sequences reported in the current study have been deposited in GenBank under accession numbers GU969060 to GU969102.
Taxonomic Coverages
Geographic Coverages
Lake Bonney, McMurdo Dry Valleys, Antarctica
West =
East =
East =
Bibliographic Citations
- Bielewicz, S., Bell, E., Kong, W., Friedberg, I., Priscu, J. C., & Morgan-Kiss, R. M. (2011). Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. The ISME Journal, 5(9), 1559–1564. - doi:10.1038/ismej.2011.23
Contacts
Scott Bielewiczoriginator
Miami University
Oxford
US
Elanor Bell
originator
Scottish Marine Institute
Argyll
GB
Weidong Kong
originator
Miami University
Oxford
US
Iddo Froedberg
originator
Miami University
Oxford
US
John Priscu
originator
Montana State University
Bozeman
US
Rachael Morgan-Kiss
originator
Miami University
Oxford
US
Maxime Sweetlove
metadata author
Royal Belgian Institute of Natural Sciences
Brussels
BE
email: msweetlove@naturalsciences.be
Rachael Morgan-Kiss
user
Miami University
Oxford
US
Rachael Morgan-Kiss
administrative point of contact
Miami University
Oxford
US