Species composition and distribution of anopheles gambiae complex circulating in Kinshasa

Study Extent

Sampling

Quality Control

Method steps

1. In each site, ten households were selected (by agreement of the heads of households) for collection of adult mosquitoes by CDC light trap. This collection was carried out over night between 18:00hrs and 06:00hrs once a month, throughout the entire study period. In each household, two traps were used, one placed inside and the other placed outside. The outdoor traps were placed within five metres of the front door. To optimise the genetic diversity of the Culicidae species collected in each study site, households selected for capture of Culicidae by CDC light trap were sampled along a 15 km long transect. The distribution of ten selected households per site followed the following pattern: three households between 0-3 km; three households between 6-9 km; and four households between 12-15 km. Different houses were used for each night, after obtaining the agreement of the heads of households. Culicidae captured were sent to the Bioecology and Vector Control Laboratory of the Kinshasa School of Public Health for morphological identification using the Coetzee key
2. The larval collection allowed us to identify the preferential larval sites of Anopheles spp. in the different sites of our study. In each study site, sampling was carried out twice a month during the dry season (July and August 2021) and once a month during the rainy season (between September 2021 and May 2022).

1. Anopheles gambiae
   rank: species
2. Anopheles funestus
   rank: species
3. Anopheles paludis
   rank: species
4. Anopheles nili
   rank: species