The InBIO Barcoding Initiative Database: Portuguese Snakeflies (Raphidioptera)

Study Extent

Specimens of Raphidioptera were captured in six sites, distributed across two districts of the country. Sampling was conducted from 2016 to 2019, in several field work expeditions.

Sampling

Specimens were captured from 2016 to 2019 in continental Portugal by direct search in six sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected male specimens were morphologically identified using a binocular stereoscopic microscope. Females specimens were identified using the BOLD (Barcode of Life Database) Identification System.

Quality Control

To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Method steps

1. Specimens of Raphidioptera were captured in continental Portugal from 2016 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage.
2. The specimens were morphologically identified resorting to available literature (Monserrat and Papenberg, 2015). Females were identified using the BOLD (Barcode of Life Database) Identification System.
3. DNA barcodes were obtained for all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
4. All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
5. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

This dataset is composed of data relating to 8 Snakefly (Raphidioptera) specimens belonging to three species present in Portugal. All species belong to the family Raphidiidae.

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Insecta
   rank: class
4. Raphidioptera
   rank: order
5. Raphidiidae
   rank: family

Continental Portugal.