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HBIs and sterols in zooplankton Nansen Legacy Q3

Citation

Kohlbach D, Wold A, Schmidt K, Smik L, Belt S T, Hop H, Assmy P (2022). HBIs and sterols in zooplankton Nansen Legacy Q3. Version 1.4. The Nansen Legacy Project. Sampling event dataset https://doi.org/10.21334/npolar.2022.941be8cc accessed via GBIF.org on 2025-05-24.

Description

Highly branched isoprenoids (HBIs) and sterols in pelagic zooplankton collected during Nansen Legacy seasonal cruise Q3 (5 - 27 August 2019)

Sampling Description

Study Extent

Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

Sampling

Samples of 23 zooplankton taxa including copepods, krill, amphipods, pteropods and gelatinous species were collected at six stations (P1, P2, P4, P5, P6, P7), using MIK nets (1200 μm with 500 μm cod end) and WP3 net (1000 μm) for large taxa, Macroplankton trawl for mostly Thysanoessa spp. (multiple mesh sizes along the net, tapering to 8 mm at its end), Bongo net (180 μm) and Multinet (180 μm) for copepods (all species), and WP2 net (90 μm) for small copepods. Samples were sorted into the lowest possible taxonomic level, and/or stage/size groups (where possible) onboard the ship and immediately frozen at -80 ºC.

Method steps

  1. HBIs and sterols were analysed at the University of Plymouth, UK. All samples were freeze-dried (-45 °C, 0.1 mbar, 24-48 h). Total lipids were extracted with chloroform/methanol (2:1, v/v) and cleaned with potassium chloride (0.88 %). Thereafter, samples were saponified with 20 % potassium hydroxide in water/methanol (1:9, v/v) at 70 °C for 1 h. Non-saponifiable lipids were extracted with hexane and purified by open column chromatography filled with SiO2. HBIs were eluted using hexane. The partially purified non-polar lipids containing HBIs were analysed by gas chromatography-mass spectrometry (GC-MS). Quantification of HBIs was achieved by integrating individual ion responses in single-ion monitoring mode, and normalising these to the corresponding peak area of the internal standard and an instrumental response factor obtained from purified standards (Belt et al., 2012). Sterols were eluted from the same silica column using hexane:methylacetate (4:1,v/v). Sterol fractions were derivatised using N,O-bis(trimethylsilyl)trifluoroacetamide (70 °C, 1 h) and analysed GC-MS. Individual sterols were identified by comparison of the mass spectra of their trimethylsilyl-ethers with published data (Belt et al., 2018). Belt, S.T., Brown, T.A., Sanz, P.C., and Rodriguez, A.N. (2012). Structural confirmation of the sea ice biomarker IP25 found in Arctic marine sediments. Environ. Chem. Lett. 10(2), 189-192 Belt, S.T., Brown, T.A., Smik, L., Assmy, P., and Mundy, C.J. (2018). Sterol identification in floating Arctic sea ice algal aggregates and the Antarctic sea ice diatom Berkeleya adeliensis. Org. Geochem. 118, 1-3

Taxonomic Coverages

Copepods, krill, amphipods, pteropods, cnidarians, ctenophores, chaetognaths, appendicularians
  1. Calanus glacialis
  2. Calanus hyperboreus
  3. Calanus finmarchicus
  4. Metridia longa
  5. Microcalanus spp.
  6. Pseudocalanus spp.
  7. Oithona similis
  8. Paraeuchaeta norvegica
  9. Meganyctiphanes norvegica
  10. Thysanoessa inermis
  11. Thysanoessa spp.
  12. Themisto abyssorum
  13. Themisto libellula
  14. Clione limacina
  15. Limacina helicina
  16. Bougainvillia superciliaris
  17. Catablema vesicarium
  18. Beroe cucumis
  19. Mertensia ovum
  20. Eukrohnia hamata
  21. Parasagitta elegans
  22. Sagitta maxima
  23. Oikopleura vanhoeffeni

Geographic Coverages

Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

Bibliographic Citations

  1. Kohlbach D, Hop H, Wold A, Schmidt K, Smik L, Belt ST, Keck Al-Habahbeh A, Woll M, Graeve M, Dąbrowska AM, Tatarek A, Atkinson A and Assmy P (2021) Multiple Trophic Markers Trace Dietary Carbon Sources in Barents Sea Zooplankton During Late Summer. Front. Mar. Sci. 7:610248. - doi: 10.3389/fmars.2020.610248

Contacts

Doreen Kohlbach
originator
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no
Anette Wold
originator
position: Engineer
Norwegian Polar Institute
TROMSØ
NO
email: anette.wold@npolar.no
Katrin Schmidt
originator
position: Researcher
University of Plymouth
Plymouth
GB
email: katrin.schmidt@plymouth.ac.uk
Lukas Smik
originator
position: Postdoctoral Researcher
University of Plymouth
Plymouth
GB
email: lukas.smik@plymouth.ac.uk
Simon T. Belt
originator
position: Researcher
University of Plymouth
Plymouth
GB
email: s.belt@plymouth.ac.uk
Haakon Hop
originator
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: haakon.hop@awi.de
Philipp Assmy
originator
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: philipp.assmy@npolar.no
Doreen Kohlbach
metadata author
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no
Doreen Kohlbach
author
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no
Anette Wold
author
position: Engineer
Norwegian Polar Institute
Tromsø
NO
email: anette.wold@npolar.no
Katrin Schmidt
author
position: Researcher
University of Plymouth
Plymouth
GB
email: katrin.schmidt@plymouth.ac.uk
Lukas Smik
author
position: Postdoctoral Researcher
University of Plymouth
Plymouth
GB
email: lukas.smik@plymouth.ac.uk
Simon T. Belt
author
position: Researcher
University of Plymouth
Plymouth
GB
email: lukas.smik@plymouth.ac.uk
Haakon Hop
author
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: haakon.hop@npolar.no
Philipp Assmy
author
position: Researcher
Norwegian Polar Institute
Tromsø
NO
email: philipp.assmy@npolar.no
Doreen Kohlbach
administrative point of contact
position: Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
email: doreen.kohlbach@npolar.no
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