Metabarcoding of eDNAbyss water samples
Citation
MGnify (2021). Metabarcoding of eDNAbyss water samples. Sampling event dataset https://doi.org/10.15468/a5yya7 accessed via GBIF.org on 2023-09-26.Description
Water from different depths was prefiltered in situ using Salsa pump or collected in CTD bottled before being filtered onboard to retain small cell sizes or nucleic acids (DNA/RNA). Nucleic acids were extracted from filters and submitted to a PCR-ligation protocols using 6 sets of primers pairs targeting metazoans (2 primer pairs targeting a fragment of Cytochrome Oxydase I and one of rDNA18S V1-V2), microeukaryotes (2 primer pairs targeting rDNA 18S V4 and V9) and prokaryotes (2 sets of primer pairs targeting rDNA16S V4-V5 respectively defined for prokaryotes in general, and archaebacterial in particular).Sampling Description
Sampling
Water from different depths was prefiltered in situ using Salsa pump or collected in CTD bottled before being filtered onboard to retain small cell sizes or nucleic acids (DNA/RNA). Nucleic acids were extracted from filters and submitted to a PCR-ligation protocols using 6 sets of primers pairs targeting metazoans (2 primer pairs targeting a fragment of Cytochrome Oxydase I and one of rDNA18S V1-V2), microeukaryotes (2 primer pairs targeting rDNA 18S V4 and V9) and prokaryotes (2 sets of primer pairs targeting rDNA16S V4-V5 respectively defined for prokaryotes in general, and archaebacterial in particular).Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/5.0
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
- Brandt MI, Pradillon F, Trouche B, Henry N, Liautard-Haag C, Cambon-Bonavita MA, Cueff-Gauchard V, Wincker P, Belser C, Poulain J, Arnaud-Haond S, Zeppilli D. 2021. Evaluating sediment and water sampling methods for the estimation of deep-sea biodiversity using environmental DNA. Sci Rep vol. 11 - DOI:10.1038/s41598-021-86396-8
Contacts
originatorThe French National Sequencing Center (Genoscope)
Le Ponant Building D - 25 rue Leblanc
PARIS - RCS B 775 685 019
75015
FR
Telephone: +33 (0) 1 64 50 20 59
metadata author
The French National Sequencing Center (Genoscope)
Le Ponant Building D - 25 rue Leblanc
PARIS - RCS B 775 685 019
75015
FR
Telephone: +33 (0) 1 64 50 20 59
administrative point of contact
The French National Sequencing Center (Genoscope)
Le Ponant Building D - 25 rue Leblanc
PARIS - RCS B 775 685 019
75015
FR
Telephone: +33 (0) 1 64 50 20 59