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Antarctic cryosphere fungal diversity (2015)

Citation

de Menezes G, Camara P, Pinto O, Convey P, Calvarho-Silva M, Simões J, Rosa C, Rosa L, Sweetlove M (2021). Antarctic cryosphere fungal diversity (2015). SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/wwbr57 accessed via GBIF.org on 2023-12-06.

Description

Amplicon sequencing dataset (Illumina MiSeq) targeting Fungi (ITS) in ice samples (n=8) from the Antarctic Peninsula.

Sampling Description

Study Extent

Glacial ice fragments of approximately 20 kg mass, were collected adjacent to the ice fronts of seven marine terminating glaciers in the South Shetland Islands and the north-west Antarctica Peninsula during the austral summer season in December 2015 and December 2016.

Sampling

Samples were collected using sterile suits and gloves. Each sample was broken into smaller pieces, and surface decontamination carried out using 5% sodium hypochlorite (10 s), sterilized distilled water (10 s), and exposure to ultraviolet radiation (10 min).

Method steps

  1. The samples were melted and a total of 12–15 L of the resulting water Filtered through 47 mm diameter (Millipore) membranes (three membranes per sampling site, each using 4–5 L) until each membrane became saturated. Membranes were then stored at -20°C until DNA extraction.
  2. The three membranes resulting from filtering the melted ice from each sampling site were processed together in order to increase DNA yield. Total DNA was extracted using 0.5 mL extraction buffer [sodium dodecyl sulfate (SDS) 10%], left at 55°C for 18 h, followed by 165 μL NaCl (5 M) and 165 μL cetyltrimethylammonium bromide (CTAB, 10%), then 600 μL chloroform was added and the mixture centrifuged (Eppendorf/Germany) at 13,000 rpm for 10 min. The supernatant was cleaned using the QIAGEN DNeasy PowerClean cleanup Kit. The ITS2 region was used as a DNA barcode, using the universal primers ITS3 and ITS4 and were sequenced at Macrogen Inc. (South Korea) on an Illumina MiSeq sequencer, using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s protocol.

Taxonomic Coverages

Geographic Coverages

Antarctica: maritime Antarctica.

Bibliographic Citations

  1. de Menezes, G., Câmara, P., Pinto, O., Convey, P., Carvalho-Silva, M., Simões, J., ... & Rosa, L. (2021). Fungi in the Antarctic cryosphere: using DNA metabarcoding to reveal fungal diversity in glacial ice from the Antarctic Peninsula region. -

Contacts

Graciele de Menezes
originator
Universidade Federal de Minas
BR
Paulo Camara
originator
Universidade de Brasília
BR
Otavio Pinto
originator
Universidade de Brasília
BR
Peter Convey
originator
British Antarctic Survey
GB
Micheline Calvarho-Silva
originator
Universidade de Brasília
BR
Jefferson Simões
originator
Universidade Federal do Rio Grande do Sul
BR
Carlos Rosa
originator
Universidade Federal de Minas
BR
Luiz Rosa
originator
Universidade Federal de Minas
BR
Maxime Sweetlove
metadata author
Royal Belgian Institute of Natural Sciences
Brussels
BE
email: msweetlove@naturalsciences.be
Luiz Rosa
user
Universidade Federal de Minas
BR
Luiz Rosa
administrative point of contact
Universidade Federal de Minas
BR
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