NEON Biorepository Mammal Collection (DNA Extracts)
Citation
National Ecological Observatory Network (2023). NEON Biorepository Mammal Collection (DNA Extracts). Occurrence dataset https://doi.org/10.15468/6mxmvr accessed via GBIF.org on 2023-03-31.Description
This collection contains genetic extracts from ear punch (tissue) samples collected from select small mammals during small mammal sampling (NEON sample class: mam_barcoding_in.dnaSubsampleID.mam). Small mammal sampling is based on the lunar calendar, with timing of sampling constrained to occur within 10 days before or after the new moon. Typically, core sites are sampled 6 times per year, and relocatable sites 4 times per year. Small mammals are sampled using box traps (models LFA, XLK, H.B. Sherman Traps, Inc., Tallahassee, FL, USA). Box traps are arrayed in three to eight (depending on the size of the site) 10 x 10 grids with 10m spacing between traps at all sites. Small mammal trapping bouts are comprised of one or three nights of trapping, depending on whether a grid is designated for pathogen sample collection (3 nights) or not (1 night). Up to 240 ear punches are collected from each target and opportunistic species per site per year. These tissues are preserved in an appropriate tissue vial and up to 95 tissue samples per domain will be shipped to an external lab. DNA will be extracted and target sequences amplified via PCR. Barcodes of cytochrome oxidase I will be generated per specimen. Genetic extracts in 96-well plates from the Canadian Centre for DNA Barcoding are archived in the NEON Biorepository at -80 degrees Celsius. See related links below for protocols and NEON related data products.Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
Contacts
originatorNEON Biorepository Data Portal
email: biorepo@asu.edu
homepage: https://biorepo.neonscience.org/portal/index.php
metadata author
NEON Biorepository Data Portal
email: biorepo@asu.edu
homepage: https://biorepo.neonscience.org/portal/index.php
administrative point of contact
NEON Biorepository Mammal Collection (DNA Extracts)
email: biorepo@asu.edu
homepage: https://data.neonscience.org/data-products/DP1.10076.001
NEON Biorepository
content provider
email: biorepo@asu.edu
Azhar Husain
content provider
position: Collection Manager
Laura Rocha Prado
content provider
position: Bioinformatician
email: lauraprado@asu.edu
userId: https://orcid.org/0000-0003-1237-2824