The InBIO Barcoding Initiative Database: Blattodea 01

Study Extent

Sampling

Quality Control

Method steps

1. Specimens were collected in 23 different localities of continental Portugal. Field work was carried out between 2005 and 2019, with 84% of the records made in the interval between 2015 to 2019.
2. Specimens were collected during fieldwork by direct search and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
3. Specimens were morphologically identified with the assistance of a binocular stereoscopic microscope and using the available literature.
4. DNA barcodes were sequenced from a subset of samples from each species in the dataset, selected to represent the best geographical coverage available for the species, for a total of 28 samples. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For two specimens of Reticulitermes grassei only a 325 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
5. All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Insecta
   rank: class
4. Blattodea
   rank: order
5. Blattidae
   rank: family
6. Ectobiidae
7. Rhinotermitidae