BASIDIOMYCETE YEASTS ISOLATED FROM PELTIGERA LICHENS OF SOUTHERN CHILE

Study Extent

Sampling

Method steps

1. The thalli were washed twice with sterile distilled water and subsequently macerated.
2. They were seeded on three culture media (Yeast Malt Agar, Potato Dextrose Agar and a minimum agar medium without amino acids) supplemented with antibiotics.
3. Cultures were incubated at 10°C in the dark for up to 60 days.
4. Long-term preservation of yeast isolates was achieved at -20°C using cryotubes with sterile 15% glycerol and a dehydrated gelatin drop method at room temperature with sterile 10% gelatin.
5. Taxonomic identification was performed by sequencing the ITS marker and subsequent phylogenetic analyses.

1. Fungi
   rank: kingdom
2. Basidiomycota
   rank: phylum
3. Agaricostilbomycetes
   rank: class
4. Cystobasidiomycetes
   rank: class
5. Microbotryomycetes
   rank: class
6. Tremellomycetes
   rank: class
7. Agaricostilbales
   rank: order
8. Cystobasidiales
   rank: order
9. Cystofilobasidiales
   rank: order
10. Filobasidiales
    rank: order
11. Holtermanniales
    rank: order
12. Sporidiobolales
    rank: order
13. Tremellales
    rank: order
14. Bulleraceae
    rank: family
15. Bulleribasidiaceae
    rank: family
16. Chionosphaeraceae
    rank: family
17. Cystobasidiaceae
    rank: family
18. Filobasidiaceae
    rank: family
19. Holtermanniaceae
    rank: family
20. Mrakiaceae
    rank: family
21. Piskurozymaceae
    rank: family
22. Sporidiobolaceae
    rank: family
23. Cystobasidium cf. laryngis
    rank: species
24. Cystobasidium cf. pinicola
    rank: species
25. Cystobasidium lysinophilum
    rank: species
26. Cystobasidium ongulense
    rank: species
27. Cystobasidium cf. psychroaquaticum
    rank: species
28. Filobasidium stepposum
    rank: species
29. Filobasidium wieringae
    rank: species
30. Fonsecazyma cf. betulae
    rank: species
31. Holtermanniella cf. wattica
    rank: species
32. Naganishia adeliensis
    rank: species
33. Naganishia onofrii
    rank: species
34. Cystobasidium
    rank: genus
35. Filobasidium
    rank: genus
36. Fonsecazyma
    rank: genus
37. Genolevuria
    rank: genus
38. Goffeauzyma
    rank: genus
39. Holtermanniella
    rank: genus
40. Naganishia
    rank: genus
41. Pseudotremella
    rank: genus
42. Rhodotorula
    rank: genus
43. Solicoccozyma
    rank: genus
44. Tausonia
    rank: genus
45. Rhodotorula cf. kratochvilovae
    rank: species
46. Solicoccozyma gelidoterrea
    rank: species
47. Solicoccozyma terricola
    rank: species
48. Tausonia cf. pullulans
    rank: species
49. Vishniacozyma cf. dimennae
    rank: species
50. Vishniacozyma tephrensis
    rank: species