The InBIO Barcoding Initiative Database: Ypsolopha milfontensis, a new species from the Portuguese coast (Lepidoptera: Ypsolophidae)

Study Extent

Specimens were collected in the districts of Bragança, Beja and Vila Real of continental Portugal. Sampling was conducted in 2019.

Sampling

Sampling of specimens was done at four different localities of continental Portugal. Sampling was conducted in May and September of 2019. Captured specimens were preserved in 96% ethanol or in dry storage. A tissue sample, a leg, was removed from each individual and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.

Quality Control

The specimens' morphological identification was contrasted with the BLAST results of the specimens' newly generated DNA barcodes in the BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).

Method steps

1. Sampling of specimens was done at four different localities of continental Portugal, in 2019. Adult specimens were detected by direct search and hand-netted during the day, and lured using mercury-vapor lamps or UV light traps by night. Ypsolopha milfontensis larvae were found feeding on Ephedra fragilis Desf (Gnetopsida, Ephedraceae), and reared to adulthood.
2. Captured specimens were preserved in 96% ethanol or in dry storage.
3. Specimens were morphologically identified using a binocular stereoscopic microscope and resorting to available taxonomic literature.
4. DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
5. The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).

This dataset is composed of data relating to eight Lepidoptera specimens. All specimens were determined to species level. The dataset is composed of three species, Ypsolopha alpella (Denis & Schiffermüller, 1775), Y. milfontensis Corley & Ferreira, 2021 and Y. rhinolophi Corley, 2019. All species belong to the family Ypsolophidae Guenée, 1845.

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Insecta
   rank: class
4. Lepidoptera
   rank: order
5. Ypsolophidae
   rank: family

Continental Portugal