Diversity of methyl alkyl succinate synthase genes in hydrocarbon seep sediments (Raw sequence reads)

Sampling

In this study, the potential of hydrocarbon biodegradation in marine sediments was determined through the detection of a functional biomarker, the masD gene encoding (1-methyl-alkyl) succinate synthase, the key enzyme of anaerobic alkane degradation. We studied the masD diversity in 12 sampling sites from 7 methane, gas and hydrocarbon seeps differing in water depth, sediment depth, temperature, sulfate reduction rate and hydrocarbon composition (alkanes, aromatics) by parallel 454-tagged sequencing.

Method steps

1. Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1