INIA-CRF (CSIC) Plant genetic resources for agriculture and food base- collection

Study Extent

CRF's active base included accessions collected in 118 different countries

Sampling

Plant genetics resources acquisition procedure used at the CRF follow the international standards available at: https://www.fao.org/3/i3704e/i3704e.pdf

Quality Control

Seed status regarding germination and viability is checking every ten year for most of the conserved species. Germination tests are performed according to international standards for germplasm banks.

Method steps

1. The methodology to obntais different depending on the type of material to be collected. • Collecting cultivated varieties: - Prospecting to determine the collection area - Contact with local agriculture staff and potential donors of local varieties - Schedule germplasm collecting mission to make collection at harvest time of after farmers harvest - Collect seed of crop species from farmer's fields, stores or local market - On-site collection of passport data, uses, agricultural cycles and other interesting information - Uniquely identify each sample. - Package the materials in optimal conditions until delivery to the genebank facilities. • Collection of wild species - List the species of interest - Apply, for each species and location, for the requested collection permits - Locate the distribution of these species on the basis of bibliographic information. - Establish a work schedule according to the phenological cycles of the target species in order to identify the species and to collect at the optimum stage of seed maturation. To optimise this information, it is interesting to contact local botanists. - Field seed collection of the species of interest at the optimum stage of maturity, avoiding elements in poor condition and contaminating seeds. - Collect information of passport data, including, if possible, nearby plant communities. - Uniquely identify each sample. - Package the materials in optimal conditions until delivery to the genebank facilities. A part of the seeds conserved coming from regeneration/multiplication essays carried out at CRF facilities of other colaborating's institution. In these cases, seed are obtained after the field growing cycles most accurate for each species.

1. Plantae
   rank: kingdom

CRF's active collection included accessions collected in 118 different countries