Benthic biodiversity in four pairs of Norwegian lakes

Markus Majaneva

doi:10.15468/5pmumc

Benthic biodiversity in four pairs of Norwegian lakes

Citation

Majaneva M, Grainger M (2022). Benthic biodiversity in four pairs of Norwegian lakes. Norwegian Institute for Nature Research. Occurrence dataset https://doi.org/10.15468/5pmumc accessed via GBIF.org on 2024-12-12.

Description

The aim of the project was to assess the effect of water-level regulation on diversity of lake littoral meiofauna and protists. Littoral fine-gravel bottoms of four pairs of Norwegian lakes were sampled in June and September 2021. Lakes were sampled in pairs that had similar water-quality but one of which was regulated and the other was unregulated. The bottom material was sampled at 30-40 cm depth, 1-5 m from the shoreline. Six replicate samples from each sampling point was taken by lowering a 25-cm diameter cylinder (12 cm high) to the bottom and 5 spoons of the bottom material from inside the cylinder was transferred to each sample bottle. No macroinvertebrates (size > 1 mm) were observed in the spoons before emptying them to the sample bottles. Water was filtrated out of the bottles using a 10 µm mesh, and then, samples were stored in 96-% ethanol. In total, the dataset contains 101 samples, including 5 control samples and 96 samples from the lakes. Taxonomic composition of bottom-dwelling meiofauna and protists was determined using DNA metabarcoding of the V4 fragment of the 18S rRNA gene. Taxa were identified using the PR2 reference database (protists) and NCBI GenBank nucleotide database (meiofauna). Caution should be exercised when interpreting occurrences of single species, as the DNA metabarcoding and bioinformatics may contain errors. The effect of regulation on species richness and change in community composition (Alpha-diversity) was assessed using mixed effects models.

Sampling Description

Study Extent

Trøndelag County Norway, in the Meråker, Orkland, Melhus, Leksvik, Inderøy, Fosen and Skaun Municipalities

Sampling

Lakes were sampled in pairs that had similar water-quality but one of which was regulated and the other was unregulated. The bottom material was sampled at 30-40 cm depth, 1-5 m from the shoreline. Six replicate samples from each sampling point was taken by lowering a 25-cm diameter cylinder (12 cm high) to the bottom and 5 spoons of the bottom material from inside the cylinder was transferred to each sample bottle. No macroinvertebrates (size > 1 mm) were observed in the spoons before emptying them to the sample bottles. Water was filtrated out of the bottles using a 10 µm mesh, and then, samples were stored in 96-% ethanol.

Method steps

Taxonomic composition of bottom-dwelling meiofauna and protists was determined using DNA metabarcoding of the V4 fragment of the 18S rRNA gene. Taxa were identified using the PR2 reference database (protists) and NCBI GenBank nucleotide database (meiofauna).

Taxonomic Coverages

Benthic Invertebrates

Apicomplexa
rank: phylum
Apusomonadidae
rank: phylum
Centroheliozoa
rank: phylum
Cercozoa
rank: phylum
Chlorophyta
rank: phylum
Choanoflagellida
rank: phylum
Chrompodellids
rank: phylum
Ciliophora
rank: phylum
Conosa
rank: phylum
Cryptophyta
rank: phylum
Dinoflagellata
rank: phylum
Discoba
rank: phylum
Fungi
rank: phylum
Glaucophyta
rank: phylum
Haptophyta
rank: phylum
Hemimastigophora
rank: phylum
Hilomonadea
rank: phylum
Katablepharidophyta
rank: phylum
Lobosa
rank: phylum
Mesomycetozoa
rank: phylum
Metamonada
rank: phylum
Metazoa
rank: phylum
Ochrophyta
rank: phylum
Opalozoa
rank: phylum
Perkinsea
rank: phylum
Pseudofungi
rank: phylum
Rhodophyta
rank: phylum
Sagenista
rank: phylum
Stramenopiles
rank: phylum
Streptophyta
rank: phylum
Telonemia
rank: phylum

Geographic Coverages

Meråker, Orkland, Melhus, Leksvik, Inderåy, Fosen and Skaun Municipalities in Trøndelag County, Norway

Bibliographic Citations

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Contacts

Markus Majaneva
originator
position: Researcher
NINA
Trondheim
NO
email: markus.majaneva@nina.no
userId: http://orcid.org/0000-0003-3884-2548

Matthew Grainger
metadata author
position: Researcher
NINA
Trondheim
NO
email: matthew.grainger@nina.no
userId: http://orcid.org/0000-0001-8426-6495

Roald Vang
administrative point of contact
position: Section manager
NINA
Trondheim
NO
email: rv@nina.no
userId: http://orcid.org/0000-0002-4006-8689

Markus Majaneva
administrative point of contact
position: Researcher
NINA
Trondheim
NO
email: markus.majaneva@nina.no
userId: http://orcid.org/0000-0003-3884-2548