Microbial consortium enriched at the cathode of a solar microbial fuel cell
Citation
MGnify (2021). Microbial consortium enriched at the cathode of a solar microbial fuel cell. Sampling event dataset https://doi.org/10.15468/g37h8m accessed via GBIF.org on 2024-10-04.Description
The objective of this project is to study a microbial biocathode consortium enriched at the U.S. Naval Research Laboratory's Center for Bio/Molecular Science & Engineering. The initial inoculum for the solar microbial fuel cell was seawater collected near Rutgers, NJ, and the community has subsequently been maintained electroautotrophically via serial transfers on cathodes poised at 310 mV vs. SHE. This microbial consortium contains autotrophic organisms which can use a cathode as an electron donor and O2 as an electron acceptor, and has electrochemical properties potentially useful in bioenergy applications including microbial electrosynthesis and microbial fuel cell technology. System biology approaches will be used to identify functional genes that are relevant for bioenergy production, such as fixing CO2 as sole carbon source, electrosynthesis, O2 tolerance, etc. Several heterotrophic constituents have been isolated in pure culture, and have been sequenced by either Illumina or PacBio technologies. Furthermore, closed genomes and methylation data were obtained from PacBio sequencing of metagenomic DNA. Metagenomic libraries generated from eight biological replicates of an enriched biocathode microbial community were sequenced at 2x100 base pairs (bp) (paired-end reads) using an Illumina HiSeq 2000. The reads were assembled using either IDBAUD or Ray. The ideal k-mer length and node coverage were selected based on which gave results most similar to de novo sequencing of isolated bacterial cultures from the enriched community (Marinobacter sp. strain CP1 and Labrenzia sp. strain CP4). Metatranscriptomic libraries were generated from four reactors inoculated with the same inoculum were grown at a set potential of 310 mV SHE until current density stabilized, cyclic voltammetry was recorded, and then two of them were set at a more positive potential (470 mV) and two remained at 310 mV for 48 hours before samples were harvested for RNA extraction. This experiment was repeated with four more reactors inoculated with a new cell suspension from the same source electrode for a total of four biological replicates at each potential.Sampling Description
Sampling
The objective of this project is to study a microbial biocathode consortium enriched at the U.S. Naval Research Laboratory's Center for Bio/Molecular Science & Engineering. The initial inoculum for the solar microbial fuel cell was seawater collected near Rutgers, NJ, and the community has subsequently been maintained electroautotrophically via serial transfers on cathodes poised at 310 mV vs. SHE. This microbial consortium contains autotrophic organisms which can use a cathode as an electron donor and O2 as an electron acceptor, and has electrochemical properties potentially useful in bioenergy applications including microbial electrosynthesis and microbial fuel cell technology. System biology approaches will be used to identify functional genes that are relevant for bioenergy production, such as fixing CO2 as sole carbon source, electrosynthesis, O2 tolerance, etc. Several heterotrophic constituents have been isolated in pure culture, and have been sequenced by either Illumina or PacBio technologies. Furthermore, closed genomes and methylation data were obtained from PacBio sequencing of metagenomic DNA. Metagenomic libraries generated from eight biological replicates of an enriched biocathode microbial community were sequenced at 2x100 base pairs (bp) (paired-end reads) using an Illumina HiSeq 2000. The reads were assembled using either IDBAUD or Ray. The ideal k-mer length and node coverage were selected based on which gave results most similar to de novo sequencing of isolated bacterial cultures from the enriched community (Marinobacter sp. strain CP1 and Labrenzia sp. strain CP4). Metatranscriptomic libraries were generated from four reactors inoculated with the same inoculum were grown at a set potential of 310 mV SHE until current density stabilized, cyclic voltammetry was recorded, and then two of them were set at a more positive potential (470 mV) and two remained at 310 mV for 48 hours before samples were harvested for RNA extraction. This experiment was repeated with four more reactors inoculated with a new cell suspension from the same source electrode for a total of four biological replicates at each potential.Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/5.0
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
- Malanoski AP, Lin B, Eddie BJ, Wang Z, Hervey WJ, Glaven SM. 2018. Relative abundance of 'Candidatus Tenderia electrophaga' is linked to cathodic current in an aerobic biocathode community. Microb Biotechnol vol. 11 - DOI:10.1111/1751-7915.12757
- Eddie BJ, Wang Z, Hervey WJ, Leary DH, Malanoski AP, Tender LM, Lin B, Strycharz-Glaven SM. 2017. Metatranscriptomics Supports the Mechanism for Biocathode Electroautotrophy by " Tenderia electrophaga". mSystems vol. 2 - DOI:10.1128/msystems.00002-17
- Wang Z, Eddie BJ, Malanoski AP, Hervey WJ, Lin B, Strycharz-Glaven SM. 2016. Complete Genome Sequence of Labrenzia sp. Strain CP4, Isolated from a Self-Regenerating Biocathode Biofilm. Genome Announc vol. 4 - DOI:10.1128/genomea.00354-16
- Wang Z, Eddie BJ, Malanoski AP, Hervey WJ, Lin B, Strycharz-Glaven SM. 2015. Complete Genome Sequence of Marinobacter sp. CP1, Isolated from a Self-Regenerating Biocathode Biofilm. Genome Announc vol. 3 - DOI:10.1128/genomea.01103-15
- Leary DH, Hervey WJ, Malanoski AP, Wang Z, Eddie BJ, Tender GS, Vora GJ, Tender LM, Lin B, Strycharz-Glaven SM. 2015. Metaproteomic evidence of changes in protein expression following a change in electrode potential in a robust biocathode microbiome. Proteomics vol. 15 - DOI:10.1002/pmic.201400585
- Wang Z, Leary DH, Malanoski AP, Li RW, Hervey WJ, Eddie BJ, Tender GS, Yanosky SG, Vora GJ, Tender LM, Lin B, Strycharz-Glaven SM. 2015. A previously uncharacterized, nonphotosynthetic member of the Chromatiaceae is the primary CO2-fixing constituent in a self-regenerating biocathode. Appl Environ Microbiol vol. 81 - DOI:10.1128/aem.02947-14
Contacts
originatorUS Naval Research laboratory
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US Naval Research laboratory
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US Naval Research laboratory