The InBIO Barcoding Initiative Database: Borkhausenia crimnodes Meyrick, 1912 (Lepidoptera, Oecophoridae), a southern hemisphere species resident in Portugal

Study Extent

Sampling

Quality Control

Method steps

1. The Borkhausenia crimnodes specimen was captured in a single locality of continental Portugal, during 2014.
2. The specimen was captured by direct search of the environment. The captured specimen was preserved in 96% ethanol.
3. The specimen was morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Meyrick, 1912).
4. A DNA barcode was sequenced from a tissue sample, one leg, that was removed. DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced.
5. The obtained sequence was submitted to BOLD and GenBank databases and the specimen morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Insecta
   rank: class
4. Lepidoptera
   rank: order
5. Oecophoridae
   rank: family