We’re sorry, but GBIF doesn’t work properly without JavaScript enabled.
Our website has detected that you are using an outdated insecure browser that will prevent you from using the site. We suggest you upgrade to a modern browser.
{{nav.loginGreeting}}
  • Get data
      • Occurrences
      • GBIF API
      • Species
      • Datasets
      • Occurrence snapshots
      • Hosted portals
      • Trends
  • How-to
    • Share data

      • Quick-start guide
      • Dataset classes
      • Data hosting
      • Standards
      • Become a publisher
      • Data quality
      • Data papers
    • Use data

      • Featured data use
      • Citation guidelines
      • GBIF citations
      • Citation widget
  • Tools
    • Publishing

      • IPT
      • Data validator
      • Scientific Collections
      • Suggest a dataset
      • New data model ⭐️
    • Data access and use

      • Hosted portals
      • Data processing
      • Derived datasets
      • rgbif
      • pygbif
      • MAXENT
      • Tools catalogue
    • GBIF labs

      • Species matching
      • Name parser
      • Sequence ID
      • Relative observation trends
      • GBIF data blog
  • Community
    • Network

      • Participant network
      • Nodes
      • Publishers
      • Network contacts
      • Community forum
      • alliance for biodiversity knowledge
    • Volunteers

      • Mentors
      • Ambassadors
      • Translators
      • Citizen scientists
    • Activities

      • Capacity enhancement
      • Programmes & projects
      • Training and learning resources
      • Data Use Club
      • Living Atlases
  • About
    • Inside GBIF

      • What is GBIF?
      • Become a member
      • Governance
      • Implementation plan
      • Work Programme
      • Funders
      • Partnerships
      • Release notes
      • Contacts
    • News & outreach

      • News
      • Newsletters and lists
      • Events
      • Ebbe Nielsen Challenge
      • Graduate Researchers Award
      • Science Review
      • Data use
  • User profile

Planktonic bacterial communities in the arctic Canada Basin and Kongsfjorden

Dataset homepage

Citation

MGnify (2019). Planktonic bacterial communities in the arctic Canada Basin and Kongsfjorden. Sampling event dataset https://doi.org/10.15468/thsffx accessed via GBIF.org on 2023-02-09.

Description

Planktonic bacteria play key role in biogeochemical cycles and energy flow in marine ecosystems. However, our knowledge of the extent and character of bacterial diversity in polar marine environments is still limited. Here we present the use of high throughput DNA pyrosequencing and statistical inference to assess the diversity of planktonic bacteria in the arctic Canada Basin, and Kongsfjorden in Spitsbergen. The V3 region of the 16S rRNA gene was amplified from seawater samples collected from the upper water column. The number of bacterial 16S rRNA sequences obtained from each sites varied from 7433 to 9218. In the Canada Basin, the most abundant bacterial groups in two sites of water at 10m depth were the Proteobacteria (mainly Alphaproteobacteria), Actinobacteria, Bacteroidetes and Cyanobacteria. Different bacterial communities were observed in the deeper water (80 or 100m depth) at the two sites. The Proteobacteria and Bacteroidetes also formed the dominant bacterial groups in surface water of Kongsfjorden. However, Gammaproteobacteria accounted for more than 90% of the Proteobacteria.

Sampling Description

Sampling

Planktonic bacteria play key role in biogeochemical cycles and energy flow in marine ecosystems. However, our knowledge of the extent and character of bacterial diversity in polar marine environments is still limited. Here we present the use of high throughput DNA pyrosequencing and statistical inference to assess the diversity of planktonic bacteria in the arctic Canada Basin, and Kongsfjorden in Spitsbergen. The V3 region of the 16S rRNA gene was amplified from seawater samples collected from the upper water column. The number of bacterial 16S rRNA sequences obtained from each sites varied from 7433 to 9218. In the Canada Basin, the most abundant bacterial groups in two sites of water at 10m depth were the Proteobacteria (mainly Alphaproteobacteria), Actinobacteria, Bacteroidetes and Cyanobacteria. Different bacterial communities were observed in the deeper water (80 or 100m depth) at the two sites. The Proteobacteria and Bacteroidetes also formed the dominant bacterial groups in surface water of Kongsfjorden. However, Gammaproteobacteria accounted for more than 90% of the Proteobacteria.

Method steps

  1. Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1

Taxonomic Coverages

Geographic Coverages

Bibliographic Citations

Contacts

originator
PRIC
metadata author
PRIC
administrative point of contact
PRIC
What is GBIF? API FAQ Newsletter Privacy Terms and agreements Citation Code of Conduct Acknowledgements
Contact GBIF Secretariat Universitetsparken 15 DK-2100 Copenhagen Ø Denmark