The InBIO Barcoding Initiative Database: Mondeguina, a new genus for Apatetris mediterranella Nel & Varenne, 2012, with description of a new species from Portugal (Lepidoptera, Gelechiidae)

Study Extent

Sampling

Quality Control

Method steps

1. Sampling of specimens was done at three different localities of continental Portugal, between 2015 and 2018. Specimens were detected and captured at night by using mercury-vapor lamps as lures, although part of the specimens were collected a few metres away from the light, resting on grasses.
2. The captured specimens were preserved in 96% ethanol.
3. The specimens were morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley, Rosete & Ferreira, 2017; Gozmány, 1954; Janse, 1949–1954, Junnilainen & Nupponen, 2010; Nel & Varenne, 2012; Sakamaki, 2000; Staudinger, 1880).
4. DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
5. The obtained sequences were submitted to BOLD database and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Insecta
   rank: class
4. Lepidoptera
   rank: order
5. Gelechiidae
   rank: family