Rhizobacteria promoting plant growth and bacteria degrading toxic pollutants (CNR-IBBR-SUB)

Carmen Bianco; Roberto Defez

doi:10.15468/bprp46

Rhizobacteria promoting plant growth and bacteria degrading toxic pollutants (CNR-IBBR-SUB)

Citation

Bianco C, Defez R, Bucci G (2024). Rhizobacteria promoting plant growth and bacteria degrading toxic pollutants (CNR-IBBR-SUB). Version 1.12. Consiglio Nazionale delle Ricerche, Istituto di Bioscienze e BioRisorse (CNR-IBBR). Occurrence dataset https://doi.org/10.15468/bprp46 accessed via GBIF.org on 2024-12-12.

Description

The dataset includes metadata of endophytic bacteria with plant growth promoting activity and bacteria usable for bio-remedation of polluted environments from the CNR-IBBR-SUB collection, which is maintained at Institute of Biosciences and Bioresources (IBBR) in Naples, Italy. Endophytic bacteria were isolated from rice plants grown in Italy and in the State of Mali (West Africa), while polycyclic aromatic hydrocarbon degrading bacteria were isolated from the industrial zone of the port of Marghera (Venice, Italy). Microorganisms are cryopreserved at -80 °C resuspended in growth media containing cryoprotectants (glycerol). Frozen microorganisms can be revived by thawing at 37 °C and inoculating into fresh culture medium. The main aim of the collection is to study bacteria interacting with legumes and cereals (e.g., N-fixation, phytohormones production, etc.) and their ability to improve host plants resistance to abiotic stresses.

Purpose

Microbiology; genomics; metabolomics. Microbiology: growth and manipulation of bacteria. Molecular Biology: isolation and manipulation of nucleic acids; cloning and heterologous expression of genes of interest in model organisms (E. coli); gene expression analysis (RT-PCR, RNA-seq). Biochemistry: structural and functional analysis of proteins by SDS-PAGE, native-PAGE and western blot; UV-visible, fluorescent and circular dichroism spectroscopy; enzymatic assays (varius enzymes inclucing DNA polymerases); in vitro DNA-ligand interaction techniques by innovative techniques, as the Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI); fluorescence microscopy for in vivo study of bacteria, nematodes, and plants.

Sampling Description

Method steps

Taxonomic Coverages

The following families are represented: Enterobacterales, Sphingobacteriales, Pseudomonadales, Flavobacteriales, Xanthomonadales, Actinomycetales.

Paenibacillales
rank: order
Sphingobacteriales
rank: order
Micrococcaceae
rank: family
Sphingobacteriaceae
rank: family
Firmicutes
rank: phylum
Actinomycetia
rank: class
Xanthomonadaceae
rank: family
Bacilli
rank: class
Paenibacillaceae
rank: family
Weeksellaceae
rank: family
Gammaproteobacteria
rank: class
Exiguobacteraceae
rank: family
Actinomycetales
rank: order
Xanthomonadales
rank: order
Bacteroidota
rank: phylum
Pseudomonadales
rank: order
Aeromonadaceae
rank: family
Bacillaceae
rank: family
Exiguobacterales
rank: order
Proteobacteria
rank: phylum
Flavobacteriales
rank: order
Shewanellaceae
rank: family
Actinobacteriota
rank: phylum
Microbacteriaceae
rank: family
Bacteroidia
rank: class
Burkholderiaceae
rank: family
Pseudomonadaceae
rank: family
Bacteria
rank: kingdom
Enterobacteriaceae
rank: family
Bacillales
rank: order
Enterobacterales
rank: order
Moraxellaceae
rank: family
Burkholderiales
rank: order

Geographic Coverages

Italy, Europe and Mali, Western-Central Africa

Bibliographic Citations

Bianco C, Andreozzi A, Romano S, Fagorzi C, Cangioli L, Prieto P, Cisse F, Niangado O, Sidibé A, Pianezze S, et al. (2021). Endophytes from African Rice (Oryza glaberrima L.) efficiently colonize Asian Rice (Oryza sativa L.) stimulating the activity of its antioxidant enzymes and increasing the content of nitrogen, carbon, and chlorophyll. Microorganisms 9, 1714. - https://doi.org/10.3390/microorganisms9081714
The overproduction of indole-3-acetic acid (IAA) in endophytes up-regulates nitrogen-fixation in both bacterial cultures and inoculated rice plants. Microbial Ecology, 74, 441-452. - https://doi.org/10.1007/s00248-017-0948-4
Bianco C, Defez R (2015). Microorganisms and their use for environmental cleanup. Patent no. RM2015A00096, National Research Council of Italy, Rome, Italy. - http://www.cnr.it/ontology/cnr/individuo/prodotto/ID326828

Contacts

Carmen Bianco
originator
position: Researcher
CNR-IBBR-NA, Institute of Biosciences and Bioresources (IBBR) - Section of Naples, National Research Council of Italy
v. Pietro Castellino 111
Naples
I-80131
NA
IT
email: carmen.bianco@ibbr.cnr.it
homepage: https://ibbr.cnr.it/ibbr/info/people/carmelina-bianco
userId: https://orcid.org/0000-0002-8489-3779

Roberto Defez
originator
position: Researcher
CNR-IBBR-NA, Institute of Biosciences and Bioresources (IBBR) - Section of Naples, National Research Council of Italy
v. Pietro Castellino 111
Naples
I-80131
NA
IT
email: roberto.defez@ibbr.cnr.it
homepage: https://www.ibbr.cnr.it/ibbr/info/people/roberto-defez
userId: https://orcid.org/0000-0002-5473-7740

Gabriele Bucci
metadata author
position: Senior Researcher
CNR-IBBR, Institute of Biosciences and BioResources, National Research Council of Italy
v. Madonna del Piano 10
Sesto Fiorentino
I-50019
FI
email: gabriele.bucci@cnr.it
homepage: https://ibbr.cnr.it/info/people/gabriele-bucci
userId: https://orcid.org/0000-0001-8931-0344

Institute of Biosciences and BioResources CNR-IBBR
owner
position: Research Institute
National Research Council of Italy (CNR)
v. Piero Castellino 111
Naples
I-80131
NA
IT
email: carmelina.bianco@ibbr.cnr.it
homepage: https://biomemory.cnr.it/collections/CNR-IBBR-SUB

Carmen Bianco
administrative point of contact
position: Reference Person
CNR-IBBR-NA, Institute of Biosciences and Bioresources (IBBR) - Section of Naples
v. Pietro Castellino 111
Naples
I-80131
NA
IT
email: carmen.bianco@ibbr.cnr.it
homepage: https://ibbr.cnr.it/ibbr/info/people/carmelina-bianco
userId: https://orcid.org/0000-0002-8489-3779