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Microbial diversity in the Benguela coastal upwelling system as derived from 16S rRNA sequencing and RNA Stable Isotope Probing (SIP)

Citation

MGnify (2018). Microbial diversity in the Benguela coastal upwelling system as derived from 16S rRNA sequencing and RNA Stable Isotope Probing (SIP). Sampling event dataset https://doi.org/10.15468/7ltmwm accessed via GBIF.org on 2022-08-12.

Description

Sediment microbial diversity was assessed in samples retrieved from four sampled transects. 16S metagenomics was used to characterize microbial structure in the sediments of the different sampling stations. Furthermore, stable isotope probing was employed in samples from certain stations in an attempt to link the function and identity of the microorganisms of the sediment samples. Two microcosm experiments were conducted with one involving the addition of nitrate as substrate and the other one sulphate. In both experiments the provided electron donor was acetate.

Sampling Description

Sampling

Sediment microbial diversity was assessed in samples retrieved from four sampled transects. 16S metagenomics was used to characterize microbial structure in the sediments of the different sampling stations. Furthermore, stable isotope probing was employed in samples from certain stations in an attempt to link the function and identity of the microorganisms of the sediment samples. Two microcosm experiments were conducted with one involving the addition of nitrate as substrate and the other one sulphate. In both experiments the provided electron donor was acetate.

Method steps

  1. Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1

Taxonomic Coverages

Geographic Coverages

Bibliographic Citations

Contacts

originator
HCMR-IMBBC
metadata author
HCMR-IMBBC
administrative point of contact
HCMR-IMBBC
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