Free-living nematodes of the C1-LTER station (Gulf of Trieste, Northern Adriatic Sea), 2010-2012
Citation
Franzo A (2022): Free-living nematodes of the C1-LTER station (Gulf of Trieste, Northern Adriatic Sea), 2010-2012. v1.5. National Institute of Oceanography and Applied Geophysics. Dataset/Samplingevent. https://doi.org/10.6092/sjfy-pp98 accessed via GBIF.org on 2023-10-02.Description
Monitoring of free-living nematodes at the C1-LTER station (Gulf of Trieste, Northern Adriatic Sea), seasonal frequency from 07-2010 to 07-2012. The sampling was carried out by a KC Haps bottom corer with polycarbonate sample tubes (12.7 cm I.D. with a sample area of 127 cm2). In laboratory, one sediment core was subsampled by means of 3 cut-off plastic syringes (2.7 cm I.D., surface area of 5.72 cm2, 0-10 cm top sediment) in order to obtain three pseudoreplicates. The nematodes (38–1000 mm) were extracted from sediment subsamples by sieving each pseudoreplicate and by centrifuging the material retained by a 38 μm mesh net with Ludox HS-40 (density of 1.15–1.18 g cm−3). For each pseudoreplicate, 100-120 nematodes were randomly picked out using a fine pin under a stereomicroscope (Zeiss Discovery V20, magnification 40×), transferred from formalin to glycerol through a series of ethanol-glycerol solutions and finally mounted on permanent glass slides in anhydrous glycerin. Nematodes were identified under a microscope (100x magnification) at the genus level using dedicated pictorial and identification keys.Sampling Description
Study Extent
Sampling at seasonal frequency from 07-2010 to 07-2012.Sampling
The sampling was carried out by a KC Haps bottom corer with polycarbonate sample tubes (12.7 cm I.D. with a sample area of 127 cm2).Quality Control
Replicates were sampled and analysed to take into account biological variability.Method steps
- In laboratory, one sediment core was subsampled by means of 3 cut-off plastic syringes (2.7 cm I.D., surface area of 5.72 cm2, 0-10 cm top sediment) in order to obtain three pseudoreplicates. The nematodes (38–1000 mm) were extracted from sediment subsamples by sieving each pseudoreplicate and by centrifuging the material retained by a 38 μm mesh net with Ludox HS-40 (density of 1.15–1.18 g cm−3). For each pseudoreplicate, 100-120 nematodes were randomly picked out using a fine pin under a stereomicroscope (Zeiss Discovery V20, magnification 40×), transferred from formalin to glycerol through a series of ethanol-glycerol solutions and finally mounted on permanent glass slides in anhydrous glycerin. Nematodes were identified under a microscope (100x magnification) at the genus level using dedicated pictorial and identification keys.
Taxonomic Coverages
Free-living nematodes
-
Nematodacommon name: Nematoda rank: phylum
Geographic Coverages
Gulf of Trieste: https://marineregions.org/gazetteer.php?p=details&id=3385
Bibliographic Citations
- Franzo, A., Guilini, K., Cibic, T., & Del Negro, P. (2018). Interactions between free-living nematodes and benthic diatoms: insights from the Gulf of Trieste (northern Adriatic Sea). Mediterranean Marine Science, 19(3), 538-554. - https://doi.org/10.12681/mms.15549
Contacts
Annalisa Franzooriginator
position: Scientist
Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
Borgo Grotta Gigante 42/C
Sgonico
34010
Trieste
IT
email: afranzo@ogs.it
userId: http://orcid.org/0000-0002-4127-1558
Marina Lipizer
metadata author
position: Researcher
Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
Borgo Grotta Gigante 42/C
Sgonico
34010
TS
IT
Telephone: +39 0402140310
email: mlipizer@ogs.it
userId: http://orcid.org/0000-0001-5707-6338
Paola Del Negro
originator
position: Senior Scientist
Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
Borgo Grotta Gigante 42/C
Sgonico
34010
Trieste
IT
email: pdelnegro@ogs.it
userId: http://orcid.org/0000-0003-2465-4896
Annalisa Franzo
originator
position: Scientist
Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
Borgo Grotta Gigante 42/C
Sgonico
34010
Trieste
IT
email: afranzo@ogs.it
userId: http://orcid.org/0000-0002-4127-1558
Annalisa Franzo
administrative point of contact
position: Scientist
Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
Borgo Grotta Gigante 42/C
Sgonico
34010
Trieste
IT
email: afranzo@ogs.it
userId: http://orcid.org/0000-0002-4127-1558