California Current Cruise CCE-P1408 Process Cruise #6
Citation
MGnify (2019). California Current Cruise CCE-P1408 Process Cruise #6. Sampling event dataset https://doi.org/10.15468/qpquen accessed via GBIF.org on 2024-11-11.Description
Passive sinking of particulate organic matter (POM) is the main mechanism through which the biological pump transports surface primary production to the ocean interior. However, the contribution and variability of different biological sources to vertical export is not fully understood. In this work, we use DNA metabarcoding of 18S rRNA gene and particle interceptor traps (PITs) to characterize the taxonomic composition of particles sinking out of the photic layer in the California Current Ecosystem. The PITs included formalin-fixed and 'live' traps to investigate compositional changes associated with consumption and remineralization processes. Sequences affiliated to Radiolaria, mainly Spumellaria and Acantharia, dominated the eukaryotic assemblage in fixed-traps (90%) with Dinophyta and Metazoa making minor contributions. The prominence of Radiolaria decreased markedly in live-traps (<3%), possibly due to selective consumption by large copepods, heterotrophic nanoflagellates and amoeboid phaeodarians that were heavily enriched in these traps. These patterns were consistent across the coastal-to-open water masses surveyed, despite major differences in productivity and trophic structure of the epipelagic plankton community. Our findings indicate that in addition to more commonly considered phytoplankton and crustacean zooplankton, the role of rhizarians can be significant for POM vertical export and cycling in the CCE and potentially in other oceanic ecosystems.Sampling Description
Sampling
Passive sinking of particulate organic matter (POM) is the main mechanism through which the biological pump transports surface primary production to the ocean interior. However, the contribution and variability of different biological sources to vertical export is not fully understood. In this work, we use DNA metabarcoding of 18S rRNA gene and particle interceptor traps (PITs) to characterize the taxonomic composition of particles sinking out of the photic layer in the California Current Ecosystem. The PITs included formalin-fixed and 'live' traps to investigate compositional changes associated with consumption and remineralization processes. Sequences affiliated to Radiolaria, mainly Spumellaria and Acantharia, dominated the eukaryotic assemblage in fixed-traps (90%) with Dinophyta and Metazoa making minor contributions. The prominence of Radiolaria decreased markedly in live-traps (<3%), possibly due to selective consumption by large copepods, heterotrophic nanoflagellates and amoeboid phaeodarians that were heavily enriched in these traps. These patterns were consistent across the coastal-to-open water masses surveyed, despite major differences in productivity and trophic structure of the epipelagic plankton community. Our findings indicate that in addition to more commonly considered phytoplankton and crustacean zooplankton, the role of rhizarians can be significant for POM vertical export and cycling in the CCE and potentially in other oceanic ecosystems.Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1
Taxonomic Coverages
Geographic Coverages
Bibliographic Citations
- Gutierrez-Rodriguez A, Stukel MR, Lopes Dos Santos A, Biard T, Scharek R, Vaulot D, Landry MR, Not F. 2019. High contribution of Rhizaria (Radiolaria) to vertical export in the California Current Ecosystem revealed by DNA metabarcoding. ISME J vol. 13 - DOI:10.1038/s41396-018-0322-7
Contacts
originatorSorbonne Universite, CNRS, Station Biologique
metadata author
Sorbonne Universite, CNRS, Station Biologique
administrative point of contact
Sorbonne Universite, CNRS, Station Biologique