Stable isotope ratios and elemental contents of C, N and S in benthic organisms sampled during the PROTEKER 5 summer campaign in Kerguelen Islands (2016) - data
Citation
Le Bourg B, Saucède T, Charpentier A, Lepoint G, Gan Y, Michel L N (2022): Stable isotope ratios and elemental contents of C, N and S in benthic organisms sampled during the PROTEKER 5 summer campaign in Kerguelen Islands (2016) - data. v1.4. SCAR - AntOBIS. Dataset/Samplingevent. https://ipt.biodiversity.aq/resource?r=proteker2016-event&v=1.4 https://doi.org/10.15468/frpbz6 accessed via GBIF.org on 2024-12-09.Description
"Stable isotope ratios and elemental contents of C, N and S in benthic organisms sampled during the PROTEKER 5 summer campaign in Kerguelen Islands (2016)" is sampling event type dataset published by SCAR-AntOBIS. The report of the summer campaign (in french) is available at: https://proteker.osupytheas.fr/wp-content/uploads/2021/04/PROTEKER2016-17_rapport_xs.pdf This dataset contains records of 363 specimens from 29 taxa, based on SCUBA diving collection complemented with intertidal collections on the shore and in rock pools at low tide during the PROTEKER 5 campaign in Kerguelen Islands from 03/11/2016 to 31/12/2016. The data is published as a standardized Darwin Core Archive and includes for each sampling event an eventID, date, time, location, depth range, sampling protocol (in the event core). For each occurrence, an occurrenceID, eventID, status (present/absent), catalog number, institution code, scientific name, taxa classification, preparations are specified in the occurrence extension. Each measurement associated with a sampling event or an occurrence record will have a measurementID, measurement type, measurement value and measurement unit and their corresponding identifiers if any (in the extended measurement or fact extension). This dataset is published by SCAR-AntOBIS under the license CC-BY 4.0. We would appreciate it if you could follow the guidelines from the SCAR and IPY Data Policies (https://www.scar.org/excom-meetings/xxxi-scar-delegates-2010-buenos-aires-argentina/4563-scar-xxxi-ip04b-scar-data-policy/file/) when using the data. If you have any questions regarding this dataset, do not hesitate to contact us via the contact information provided in the metadata or via data-biodiversity-aq@naturalsciences.be. Issues with dataset can be reported at https://github.com/biodiversity-aq/data-publication/ This dataset is part of the RECTO and vERSO projects funded by the Belgian Science Policy Office (BELSPO), as well as the PROTEKER project, funded by the French Polar Institute Paul-Emile Victor (IPEV).Sampling Description
Study Extent
This dataset describes the abundance and distribution of benthic organisms of the PROTEKER 5 campaign at Kerguelen Islands between 19/11/2016 and 04/12/2016.Sampling
All samples were taken in or next to (within a few meters) coastal kelp forests in the vicinity of the Port-aux-Français scientific station. Most samples were hand collected by SCUBA divers between 5 and 13 m depth. They included benthic primary producers, large macrophytodetritus, surface sediments and biomass-dominant benthic macroinvertebrates belonging to various taxa and feeding guilds: sponges, bivalves, gastropods, sedentary and mobile polychaetes, arthropods and echinoderms. SCUBA diving sampling events were complemented with intertidal collections on the shore and in rock pools at low tide. Those samples also comprised macrophytodetritus (wrack algae) as well as invertebrate consumers. Finally, for suspended particulate organic matter (SPOM), subsurface seawater samples were taken using a Niskin bottle. Water was subsequently filtered (1.8 to 2.1 dm3) on pre-combusted (400°C, 4 h) Whatman GF/F filters, and retained SPOM was kept for analysis.Quality Control
All records were validated. - Coordinates were converted into decimal latitude and decimal longitude and plotted on map to verify the actual geographical location corresponds to its locality. - All scientific names were checked for typo and matched to the species information backbone of Worlds Register of Marine Species (http://marinespecies.org/) and LSID were assigned to each taxa as scientificNameID. - Event date was converted into ISO 8601 format.Method steps
- Sample preservation: After diving, samples were placed in oxygenated water containers and transferred to the Port-aux-Français station lab facilities where they were processed.
- Sample preparation: Samples were immediately dissected to retrieve the tissue selected for stable isotope analyses (cf. "measurementRemarks" field of the dataset), which was then dried at 60°C during 72 hours. Samples were then homogenised into powder prior to SIA using a mortar and a pestle or a mixer mill (MM301, Retsch, Haan, Germany) depending on toughness. Whenever necessary (cf. "preparations" field of the dataset), carbonates were removed from subsamples by exposing subsamples to 37 % hydrochloric acid vapor during 48 hours. Acidified subsamples were then kept at 60°C until further sample preparation.
- Stable isotope analysis: The subsamples were then precisely weighed (ca 2.5-3 mg) in 5×8 tin cups with ca 3 mg of tungsten trioxide. Their elemental composition and their stable isotope values were analyzed with an elemental analyser coupled to a continuous-flow isotope-ratio mass spectrometer. Isotopic ratios were expressed in ‰ using the widespread δ notation (Coplen 2011) and relative to the international references Vienna Pee Dee Belemnite (for carbon), Atmospheric Air (for nitrogen) and Vienna Canyon Diablo Troilite (for sulfur). Sucrose (IAEA-C-6; δ13C=−10.8 ± 0.5‰; mean ± standard deviation), ammonium sulfate (IAEA-N-1; δ15N= 0.4 ± 0.2‰; mean ± SD) and silver sulfide (IAEA-S-1; δ34S = −0.3‰) were used as primary analytical standards for stable isotope ratios. Sulfanilic acid (Sigma-Aldrich; δ13C=−25.6 ± 0.4‰; δ15N=−0.13 ± 0.4‰; δ34S = 5.9 ± 0.5‰; means ± SD) was used as a secondary analytical standard for stable isotope ratios and as elemental standard. Standard deviations on multi-batch replicate measurements of secondary and internal laboratory standards (sea star tegument), analyzed interspersed with samples (one replicate of each standard every 15 analyses), were 0.3‰ for δ13C and δ15N and 0.5‰ for δ34S.
- Instrumentation: Stable isotope ratio measurements were performed by continuous flow–elemental analysis–isotope ratio mass spectrometry (CF-EA-IRMS) at University of Liège (Belgium), using a vario MICRO cube C-N-S elemental analyzer (Elementar Analysensysteme GmbH, Hanau, Germany) coupled to an IsoPrime100 isotope ratio mass spectrometer (Isoprime, Cheadle, United Kingdom).
Taxonomic Coverages
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Animaliarank: kingdom
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Chromistarank: kingdom
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Plantaerank: kingdom
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Annelidarank: phylum
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Arthropodarank: phylum
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Chlorophytarank: phylum
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Echinodermatarank: phylum
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Molluscarank: phylum
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Ochrophytarank: phylum
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Poriferarank: phylum
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Rhodophytarank: phylum
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Asteroidearank: class
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Bivalviarank: class
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Echinoidearank: class
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Florideophyceaerank: class
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Gastropodarank: class
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Holothuroidearank: class
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Malacostracarank: class
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Phaeophyceaerank: class
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Polychaetarank: class
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Ulvophyceaerank: class
Geographic Coverages
Kerguelen Island, Gulf of Morbihan, close to Port-aux-Français scientific station
Bibliographic Citations
- Coplen, T.B. (2011), Guidelines and recommended terms for expression of stable-isotope-ratio and gas-ratio measurement results. Rapid Commun. Mass Spectrom., 25: 2538-2560. - https://doi.org/10.1002/rcm.5129
Contacts
Baptiste Le Bourgoriginator
University of Liège
BE
email: lebourg.bap@orange.fr
userId: http://orcid.org/0000-0002-1455-2020
Thomas Saucède
originator
Université Bourgogne Franche-Comté
FR
email: thomas.saucede@u-bourgogne.fr
userId: http://orcid.org/0000-0001-6056-4447
Anouk Charpentier
originator
University of Liège
BE
Gilles Lepoint
originator
University of Liège
BE
email: g.lepoint@uliege.be
userId: http://orcid.org/0000-0003-4375-0357
Yi-Ming Gan
originator
Royal Belgian Institute of Natural Sciences
BE
email: data-biodiversity-aq@naturalsciences.be
userId: http://orcid.org/0000-0001-7087-2646
Loïc N. Michel
originator
University of Liège
BE
email: loicnmichel@gmail.com
userId: http://orcid.org/0000-0003-0988-7050
Loïc N. Michel
metadata author
University of Liège
BE
email: loicnmichel@gmail.com
userId: http://orcid.org/0000-0003-0988-7050
Yi-Ming Gan
metadata author
Royal Belgian Institute of Natural Sciences
BE
email: data-biodiversity-aq@naturalsciences.be
userId: http://orcid.org/0000-0001-7087-2646
Loïc N. Michel
administrative point of contact
University of Liège
BE
email: loicnmichel@gmail.com
userId: http://orcid.org/0000-0003-0988-7050
Gilles Lepoint
administrative point of contact
University of Liège
BE
userId: http://orcid.org/0000-0003-4375-0357
Yi-Ming Gan
administrative point of contact
Royal Belgian Institute of Natural Sciences
BE
email: data-biodiversity-aq@naturalsciences.be
userId: http://orcid.org/0000-0001-7087-2646