The InBIO Barcoding Initiative Database: Diptera 01

Study Extent

Specimens were collected in 59 different localities of continental Portugal. Sampling was conducted from 2014 to 2018, in several field work expeditions. More than half of the specimens (62.6%) were collected in the year 2017.

Sampling

The studied material was collected in 59 different localities from the northern half of continental Portugal (Fig. 1, Table 1). Sampling was conducted between 2014 and 2018 on a wide range of habitats, using mainly hand-held sweep nets or direct search of specimens. Collected specimens were examined both dry and in alcohol using a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature (Suppl. material 1)

Quality Control

To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine.

Method steps

1. Specimens were collected in 59 different localities of continental Portugal. Fieldwork was carried out between 2014 and 2018.
2. Specimens were collected during fieldwork by direct search of specimens or by the use of entomological nets to intercept specimens flight or to sweep the vegetation. Captured specimens were preserved in 96% alcohol.
3. Specimens were morphologically identified with the assistance of a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and using available literature.
4. DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. Specimens and DNA extracts were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
5. All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

This dataset is composed of data relating to 203 Diptera specimens. All specimens were determined to species level. Overall, 154 species are represented in the dataset. These species belong to 41 families. Five families account for 56% of the total collected specimens, Syrphidae, Muscidae, Tachinidae, Calliphoridae and Chloropidae. These five families account for 54% of the total species represented. Nineteen families are represented by a single species.

1. Animalia
   rank: kingdom
2. Arthropoda
   rank: phylum
3. Insecta
   rank: class
4. Diptera
   rank: order
5. Anisopodidae
   rank: family
6. Anthomyiidae
   rank: family
7. Asilidae
   rank: family
8. Bibionidae
   rank: family
9. Bombyliidae
   rank: family
10. Calliphoridae
    rank: family
11. Canacidae
    rank: family
12. Chloropidae
    rank: family
13. Conopidae
    rank: family
14. Diastatidae
    rank: family
15. Dolichopodidae
    rank: family
16. Drosophilidae
    rank: family
17. Ephydridae
    rank: family
18. Fanniidae
    rank: family
19. Heleomyzidae
    rank: family
20. Hippoboscidae
    rank: family
21. Hybotidae
    rank: family
22. Lauxaniidae
    rank: family
23. Lonchaeidae
    rank: family
24. Micropezidae
    rank: family
25. Muscidae
    rank: family
26. Nemestrinidae
    rank: family
27. Opetiidae
    rank: family
28. Opomyzidae
    rank: family
29. Pallopteridae
    rank: family
30. Periscelididae
    rank: family
31. Platystomatidae
    rank: family
32. Psychodidae
    rank: family
33. Ptychopteridae
    rank: family
34. Rhiniidae
    rank: family
35. Rhinophoridae
    rank: family
36. Sarcophagidae
    rank: family
37. Scathophagidae
    rank: family
38. Sciomyzidae
    rank: family
39. Stratiomyidae
    rank: family
40. Syrphidae
    rank: family
41. Tabanidae
    rank: family
42. Tachinidae
    rank: family
43. Tephritidae
    rank: family
44. Trichoceridae
    rank: family
45. Ulidiidae
    rank: family

Continental Portugal