Marine sediment microbial communities in the presence of macrophytes
Citation
MGnify (2019). Marine sediment microbial communities in the presence of macrophytes. Sampling event dataset https://doi.org/10.15468/w6rajz accessed via GBIF.org on 2024-12-13.Description
In this study we examined the sediment microbial communities in the absence and presence of two marine macrophytes (Zostera muelleri - Zm, and Caulerpa taxifolia - Ct), and at low (L) and high (H) densities of the macrophytes.Three independent and randomly obtained samples were taken from each of the five treatment groups (absent, Zm-L, Zm-H, Ct-L, Zm-H), genomic DNA extracted and PCR conducted targeting the V4 region of the 16S gene. PCR amplicons were sequenced using Illumina MiSeq 2x250 bp, with paired-end sequencing.Sampling Description
Sampling
In this study we examined the sediment microbial communities in the absence and presence of two marine macrophytes (Zostera muelleri - Zm, and Caulerpa taxifolia - Ct), and at low (L) and high (H) densities of the macrophytes.Three independent and randomly obtained samples were taken from each of the five treatment groups (absent, Zm-L, Zm-H, Ct-L, Zm-H), genomic DNA extracted and PCR conducted targeting the V4 region of the 16S gene. PCR amplicons were sequenced using Illumina MiSeq 2x250 bp, with paired-end sequencing.Method steps
- Pipeline used: https://www.ebi.ac.uk/metagenomics/pipelines/4.1
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originatorUniveristy of New South Wales
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Univeristy of New South Wales
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Univeristy of New South Wales